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Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus

The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids resulting in the production of their correspo...

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Bibliographic Details
Published in:Data in brief 2024-02, Vol.52, p.110017-110017, Article 110017
Main Authors: Orlygsson, Johann, Scully, Sean Michael
Format: Article
Language:English
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Summary:The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids resulting in the production of their corresponding branched-chain fatty acids and branched-chain alcohols with the fatty acids always being the dominant product. The highest amounts of 2-methyl-1-butanol from isoleucine were at pH 6.5, liquid-gas ratio of 0.98, and at 20 mM thiosulfate concentration. A kinetic experiment of the branched-chain amino acids was done in the presence of thiosulfate as are data on selected enzyme activities related to alcohols and aldehydes. Finally, an NMR study using 13C1 methyl-1-butyrate during the degradation of leucine in the presence of thiosulfate was done to prove that the 13C1-methyl-1-butanol was indeed from its corresponding fatty acid.
ISSN:2352-3409
2352-3409
DOI:10.1016/j.dib.2023.110017