Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells

Periodontitis is a prevalent chronic inflammatory disease triggered by a synergistic and dysbiotic microbiota present in the oral biofilm. This in vitro study is aimed at evaluating the regulation of cyclooxygenase (COX)2 expression and production by the periodontopathogen Filifactor alocis in human...

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Bibliographic Details
Published in:Mediators of inflammation 2020, Vol.2020 (2020), p.1-8
Main Authors: Eick, Sigrun, Nietzsche, Sandor, Nogueira, Andressa Vilas Boas, Nokhbehsaim, Marjan, Deschner, James
Format: Article
Language:English
Subjects:
Analysis
Biofilms
Cell culture
COX-2 inhibitors
Cyclooxygenase-2
Disease
Enzyme-linked immunosorbent assay
Enzymes
Fibroblasts
Gene expression
Inflammatory diseases
MAP kinase
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Monocytes
Penicillin
Periodontitis
Proteins
Scanning electron microscopy
Statistical analysis
TLR2 protein
Toll-like receptors
Transcription
Tumor necrosis factor
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:Periodontitis is a prevalent chronic inflammatory disease triggered by a synergistic and dysbiotic microbiota present in the oral biofilm. This in vitro study is aimed at evaluating the regulation of cyclooxygenase (COX)2 expression and production by the periodontopathogen Filifactor alocis in human gingival fibroblastic (HGF-1) and monocytic (THP-1) cells and also at investigating the underlying cellular pathway mechanisms. HGF-1 and THP-1 cells were exposed either to F. alocis or to the proinflammatory cytokine tumor necrosis factor alpha (TNFα) for 1 and 2 d to examine the COX2 expression by qPCR. COX2 protein levels were evaluated by ELISA in F. alocis-stimulated cells. Both types of cells were also stimulated with a blocking toll-like receptor (TLR)2 antibody or specific inhibitors against MAPKs. F. alocis significantly (p
ISSN:0962-9351
1466-1861
DOI:10.1155/2020/4185273