Mutant p53 sustains serine-glycine synthesis and essential amino acids intake promoting breast cancer growth

Reprogramming of amino acid metabolism, sustained by oncogenic signaling, is crucial for cancer cell survival under nutrient limitation. Here we discovered that missense mutant p53 oncoproteins stimulate de novo serine/glycine synthesis and essential amino acids intake, promoting breast cancer growt...

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Published in:Nature communications 2023-10, Vol.14 (1), p.6777-6777, Article 6777
Main Authors: Tombari, Camilla, Zannini, Alessandro, Bertolio, Rebecca, Pedretti, Silvia, Audano, Matteo, Triboli, Luca, Cancila, Valeria, Vacca, Davide, Caputo, Manuel, Donzelli, Sara, Segatto, Ilenia, Vodret, Simone, Piazza, Silvano, Rustighi, Alessandra, Mantovani, Fiamma, Belletti, Barbara, Baldassarre, Gustavo, Blandino, Giovanni, Tripodo, Claudio, Bicciato, Silvio, Mitro, Nico, Del Sal, Giannino
Format: Article
Language:English
Subjects:
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Amino acids
Breast cancer
Cell proliferation
Cell survival
Glycine
Humanities and Social Sciences
Metabolism
Missense mutant
multidisciplinary
Organoids
p53 Protein
Science
Science (multidisciplinary)
Serine
Shortages
Synthesis
Tumors
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Summary:Reprogramming of amino acid metabolism, sustained by oncogenic signaling, is crucial for cancer cell survival under nutrient limitation. Here we discovered that missense mutant p53 oncoproteins stimulate de novo serine/glycine synthesis and essential amino acids intake, promoting breast cancer growth. Mechanistically, mutant p53, unlike the wild-type counterpart, induces the expression of serine-synthesis-pathway enzymes and L-type amino acid transporter 1 (LAT1)/CD98 heavy chain heterodimer. This effect is exacerbated by amino acid shortage, representing a mutant p53-dependent metabolic adaptive response. When cells suffer amino acids scarcity, mutant p53 protein is stabilized and induces metabolic alterations and an amino acid transcriptional program that sustain cancer cell proliferation. In patient-derived tumor organoids, pharmacological targeting of either serine-synthesis-pathway and LAT1-mediated transport synergizes with amino acid shortage in blunting mutant p53-dependent growth. These findings reveal vulnerabilities potentially exploitable for tackling breast tumors bearing missense TP53 mutations. Mutant p53 induces serine/glycine synthesis and essential amino acids intake. Under amino acid restriction, mutant p53 is stabilized and activates a transcriptional program that sustains a metabolic adaptive response promoting breast cancer cells growth
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-42458-1