Human Umbilical Cord Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Reduce Skin Inflammation In Vitro

The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h befor...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-12, Vol.24 (23), p.17109
Main Authors: Lin, Tzou-Yien, Chang, Tsong-Min, Tsai, Wei-Cheng, Hsieh, Yi-Ju, Wang, Li-Ting, Huang, Huey-Chun
Format: Article
Language:English
Subjects:
Aging
Aprotinin
Cell adhesion & migration
Cell cycle
Cell growth
extracellular vesicle
Extracellular vesicles
Genes
Homeostasis
Inflammation
Life expectancy
NF-κB
Oxidative stress
p53
Particle size
Proteins
Senescence
SIRT1
Skin
Stem cell research
Stem cells
Transcription factors
Tumor necrosis factor
Tumor proteins
Umbilical cord
umbilical-cord-derived mesenchymal stem cells
Wound healing
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Summary:The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The pretreated UVMSC-EVs showed protective activity, elevating cell viability, reducing intracellular ROS, and reducing the changes in the mitochondrial membrane potential compared to the cells with a direct oxazolone treatment alone. The UCMSC-EVs exhibited anti-inflammatory activity via reducing the inflammatory cytokines IL-1β and TNF-α. A mechanism study showed that the UCMSC-EVs increased the protein expression levels of SIRT1 and P53 and reduced P65 protein expression. It was concluded that UVMSC-EVs can induce the antioxidant defense systems of HaCaT cells and that they may have potential as functional ingredients in anti-aging cosmetics for skin care.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242317109