Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy

Although the incidence and mortality rates of colorectal cancer exhibit significant variability, it remains one of the most prevalent cancers worldwide. Endeavors to prevent colorectal cancer development focus on detecting precursor lesions during colonoscopy. The diagnosis of endoscopically resecte...

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Bibliographic Details
Published in:Frontiers in oncology 2024, Vol.14, p.1394493
Main Authors: Florea, Maria-Alexandra, Eftimie, Lucian George, Glogojeanu, Remus Relu, Hristu, Radu, Stanciu, George A, Costache, Mariana
Format: Article
Language:English
Subjects:
collagen
malignant polyps
multiphoton microscopy
pseudoinvasion
second harmonic generation
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Summary:Although the incidence and mortality rates of colorectal cancer exhibit significant variability, it remains one of the most prevalent cancers worldwide. Endeavors to prevent colorectal cancer development focus on detecting precursor lesions during colonoscopy. The diagnosis of endoscopically resected polyps relies on hematoxylin and eosin staining examination. For challenging cases like adenomatous polyps with epithelial misplacement, additional diagnostic methods could prove beneficial. This paper aims to underscore stromal changes observed in malignant polyps and polyps with pseudoinvasion, leveraging two-photon excitation microscopy (TPEM), a technique extensively employed in the medical field in recent years. Both the subjective and quantitative analysis of TPEM images revealed distinct distributions and densities of collagen at the invasion front in malignant polyps compared to areas of pseudoinvasion. TPEM holds potential in discerning true invasion in malignant polyps from pseudoinvasion, offering enhanced visualization of local stromal changes.
ISSN:2234-943X
2234-943X
DOI:10.3389/fonc.2024.1394493