Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants

-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an -based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient ge...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-10, Vol.22 (19), p.10882
Main Authors: Kaur, Maninder, Manchanda, Pooja, Kalia, Anu, Ahmed, Farah K, Nepovimova, Eugenie, Kuca, Kamil, Abd-Elsalam, Kamel A
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Agrobacterium
Agrobacterium - genetics
Antibiotics
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9
Crops, Agricultural - genetics
Crops, Agricultural - metabolism
Deoxyribonucleic acid
DNA
Efficiency
Flowers & plants
Gene Editing - methods
Gene expression
Gene Expression Regulation, Plant
Genes
Genetic engineering
Genetic transformation
Genome editing
Genome, Plant
Genomes
Methods
Mutagenesis
Plant bacterial diseases
Plant Proteins - genetics
Plants - genetics
Plants - metabolism
Polyethylene glycol
Review
T-DNA
targeted site modification
Transcription
transfer-DNA
Transformation, Genetic
Transformations
transgene-free
Transgenic plants
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Description
Summary:-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an -based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on -mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms221910882