Loading…
Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland
Listeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of L. monocytogenes to animals and the food chain. The present study assessed the occurrence of L. monocytogenes in 191 sur...
Saved in:
Published in: | Scientific reports 2021-04, Vol.11 (1), p.9066-9066, Article 9066 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53 |
---|---|
cites | cdi_FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53 |
container_end_page | 9066 |
container_issue | 1 |
container_start_page | 9066 |
container_title | Scientific reports |
container_volume | 11 |
creator | Raschle, Susanne Stephan, Roger Stevens, Marc J. A. Cernela, Nicole Zurfluh, Katrin Muchaamba, Francis Nüesch-Inderbinen, Magdalena |
description | Listeria monocytogenes
is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of
L. monocytogenes
to animals and the food chain. The present study assessed the occurrence of
L. monocytogenes
in 191 surface water samples from rivers, streams and inland canals throughout Switzerland. Twenty-five (13%) of the surface water samples contained
L. monocytogenes
. Whole genome sequence (WGS) data were used to characterize the 25 isolates. The isolates belonged to major lineages I and II, with the majority assigned to either serotype 1/2a (48%), or 4b (44%). The predominant CCs identified were the hypervirulent serotype 4b clones CC1 and CC4, and the serotype CC412; all three have been implicated in listeriosis outbreaks and sporadic cases of human and animal infection worldwide. Two (8%) of the isolates belonged to CC6 which is an emerging hypervirulent clone. All isolates contained intact genes associated with invasion and infection, including
inlA/B
and
prfA
. The four CC4 isolates all harbored
Listeria
pathogenicity island 4 (LIPI-4), which confers hypervirulence. The occurrence of
L. monocytogenes
in river ecosystems may contribute to the dissemination and introduction of clinically highly relevant strains to the food chain. |
doi_str_mv | 10.1038/s41598-021-88514-y |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_edd7f122523f4b4999a486bace00a3b5</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_edd7f122523f4b4999a486bace00a3b5</doaj_id><sourcerecordid>2518853689</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53</originalsourceid><addsrcrecordid>eNp9kk1v1DAQhiMEolXpH-CAInHhkuLP2L4goapApZU4AGfL8UfqVWIvdtLV9tfjbNrScsAXWzPPvPaM36p6C8EFBJh_zARSwRuAYMM5haQ5vKhOESC0QRihl0_OJ9V5zltQFkWCQPG6OsFYAIZaeFr5q3DrUwyjDZMaauNztqMPavIx1NHVOzXdxN4Gr-uNz5NNXtVjDFEfpiVsc-1D7Ya496Gv85yc0rbeqwIeMz_2frqzaVDBvKleOTVke36_n1W_vlz9vPzWbL5_vb78vGk0ZWxqkCaIdIJo50jLsCGuxbzjWGFmS8sAM4M5MQ4ZrnALiHAdVsI5AZkhylJ8Vl2vuiaqrdwlP6p0kFF5eQzE1EuVJq8HK60xzEGEKMKOdEQIoQhvu9ICAAp3i9anVWs3d6M1ugwpqeGZ6PNM8Deyj7eSAyZazorAh3uBFH_PNk9y9FnboQzExjlLRKHAkBIqCvr-H3Qb5xTKqBaq_DFu-UKhldIp5pyse3wMBHIxhlyNIYsx5NEY8lCK3j1t47HkwQYFwCuQSyr0Nv29-z-yfwBLnMbD</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2518853689</pqid></control><display><type>article</type><title>Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland</title><source>PMC (PubMed Central)</source><source>Publicly Available Content (ProQuest)</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Raschle, Susanne ; Stephan, Roger ; Stevens, Marc J. A. ; Cernela, Nicole ; Zurfluh, Katrin ; Muchaamba, Francis ; Nüesch-Inderbinen, Magdalena</creator><creatorcontrib>Raschle, Susanne ; Stephan, Roger ; Stevens, Marc J. A. ; Cernela, Nicole ; Zurfluh, Katrin ; Muchaamba, Francis ; Nüesch-Inderbinen, Magdalena</creatorcontrib><description>Listeria monocytogenes
is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of
L. monocytogenes
to animals and the food chain. The present study assessed the occurrence of
L. monocytogenes
in 191 surface water samples from rivers, streams and inland canals throughout Switzerland. Twenty-five (13%) of the surface water samples contained
L. monocytogenes
. Whole genome sequence (WGS) data were used to characterize the 25 isolates. The isolates belonged to major lineages I and II, with the majority assigned to either serotype 1/2a (48%), or 4b (44%). The predominant CCs identified were the hypervirulent serotype 4b clones CC1 and CC4, and the serotype CC412; all three have been implicated in listeriosis outbreaks and sporadic cases of human and animal infection worldwide. Two (8%) of the isolates belonged to CC6 which is an emerging hypervirulent clone. All isolates contained intact genes associated with invasion and infection, including
inlA/B
and
prfA
. The four CC4 isolates all harbored
Listeria
pathogenicity island 4 (LIPI-4), which confers hypervirulence. The occurrence of
L. monocytogenes
in river ecosystems may contribute to the dissemination and introduction of clinically highly relevant strains to the food chain.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-88514-y</identifier><identifier>PMID: 33907261</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326 ; 631/337 ; Animals ; Aquatic ecosystems ; Aquatic environment ; Bacterial Proteins - genetics ; Ecosystem ; Food chains ; Food irradiation ; Genetic Variation ; Genome, Bacterial ; Genomes ; Genomic Islands ; Genotype ; Humanities and Social Sciences ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - pathogenicity ; Listeriosis ; Listeriosis - genetics ; Listeriosis - microbiology ; multidisciplinary ; Nucleotide sequence ; Opportunist infection ; Pathogenicity ; Pathogens ; Rivers ; Science ; Science (multidisciplinary) ; Streams ; Surface water ; Switzerland ; Water analysis ; Water sampling ; Whole Genome Sequencing</subject><ispartof>Scientific reports, 2021-04, Vol.11 (1), p.9066-9066, Article 9066</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53</citedby><cites>FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2518853689/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2518853689?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33907261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raschle, Susanne</creatorcontrib><creatorcontrib>Stephan, Roger</creatorcontrib><creatorcontrib>Stevens, Marc J. A.</creatorcontrib><creatorcontrib>Cernela, Nicole</creatorcontrib><creatorcontrib>Zurfluh, Katrin</creatorcontrib><creatorcontrib>Muchaamba, Francis</creatorcontrib><creatorcontrib>Nüesch-Inderbinen, Magdalena</creatorcontrib><title>Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Listeria monocytogenes
is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of
L. monocytogenes
to animals and the food chain. The present study assessed the occurrence of
L. monocytogenes
in 191 surface water samples from rivers, streams and inland canals throughout Switzerland. Twenty-five (13%) of the surface water samples contained
L. monocytogenes
. Whole genome sequence (WGS) data were used to characterize the 25 isolates. The isolates belonged to major lineages I and II, with the majority assigned to either serotype 1/2a (48%), or 4b (44%). The predominant CCs identified were the hypervirulent serotype 4b clones CC1 and CC4, and the serotype CC412; all three have been implicated in listeriosis outbreaks and sporadic cases of human and animal infection worldwide. Two (8%) of the isolates belonged to CC6 which is an emerging hypervirulent clone. All isolates contained intact genes associated with invasion and infection, including
inlA/B
and
prfA
. The four CC4 isolates all harbored
Listeria
pathogenicity island 4 (LIPI-4), which confers hypervirulence. The occurrence of
L. monocytogenes
in river ecosystems may contribute to the dissemination and introduction of clinically highly relevant strains to the food chain.</description><subject>631/326</subject><subject>631/337</subject><subject>Animals</subject><subject>Aquatic ecosystems</subject><subject>Aquatic environment</subject><subject>Bacterial Proteins - genetics</subject><subject>Ecosystem</subject><subject>Food chains</subject><subject>Food irradiation</subject><subject>Genetic Variation</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomic Islands</subject><subject>Genotype</subject><subject>Humanities and Social Sciences</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Listeriosis</subject><subject>Listeriosis - genetics</subject><subject>Listeriosis - microbiology</subject><subject>multidisciplinary</subject><subject>Nucleotide sequence</subject><subject>Opportunist infection</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Rivers</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Streams</subject><subject>Surface water</subject><subject>Switzerland</subject><subject>Water analysis</subject><subject>Water sampling</subject><subject>Whole Genome Sequencing</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk1v1DAQhiMEolXpH-CAInHhkuLP2L4goapApZU4AGfL8UfqVWIvdtLV9tfjbNrScsAXWzPPvPaM36p6C8EFBJh_zARSwRuAYMM5haQ5vKhOESC0QRihl0_OJ9V5zltQFkWCQPG6OsFYAIZaeFr5q3DrUwyjDZMaauNztqMPavIx1NHVOzXdxN4Gr-uNz5NNXtVjDFEfpiVsc-1D7Ya496Gv85yc0rbeqwIeMz_2frqzaVDBvKleOTVke36_n1W_vlz9vPzWbL5_vb78vGk0ZWxqkCaIdIJo50jLsCGuxbzjWGFmS8sAM4M5MQ4ZrnALiHAdVsI5AZkhylJ8Vl2vuiaqrdwlP6p0kFF5eQzE1EuVJq8HK60xzEGEKMKOdEQIoQhvu9ICAAp3i9anVWs3d6M1ugwpqeGZ6PNM8Deyj7eSAyZazorAh3uBFH_PNk9y9FnboQzExjlLRKHAkBIqCvr-H3Qb5xTKqBaq_DFu-UKhldIp5pyse3wMBHIxhlyNIYsx5NEY8lCK3j1t47HkwQYFwCuQSyr0Nv29-z-yfwBLnMbD</recordid><startdate>20210427</startdate><enddate>20210427</enddate><creator>Raschle, Susanne</creator><creator>Stephan, Roger</creator><creator>Stevens, Marc J. A.</creator><creator>Cernela, Nicole</creator><creator>Zurfluh, Katrin</creator><creator>Muchaamba, Francis</creator><creator>Nüesch-Inderbinen, Magdalena</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210427</creationdate><title>Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland</title><author>Raschle, Susanne ; Stephan, Roger ; Stevens, Marc J. A. ; Cernela, Nicole ; Zurfluh, Katrin ; Muchaamba, Francis ; Nüesch-Inderbinen, Magdalena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/326</topic><topic>631/337</topic><topic>Animals</topic><topic>Aquatic ecosystems</topic><topic>Aquatic environment</topic><topic>Bacterial Proteins - genetics</topic><topic>Ecosystem</topic><topic>Food chains</topic><topic>Food irradiation</topic><topic>Genetic Variation</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomic Islands</topic><topic>Genotype</topic><topic>Humanities and Social Sciences</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Listeriosis</topic><topic>Listeriosis - genetics</topic><topic>Listeriosis - microbiology</topic><topic>multidisciplinary</topic><topic>Nucleotide sequence</topic><topic>Opportunist infection</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Rivers</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Streams</topic><topic>Surface water</topic><topic>Switzerland</topic><topic>Water analysis</topic><topic>Water sampling</topic><topic>Whole Genome Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raschle, Susanne</creatorcontrib><creatorcontrib>Stephan, Roger</creatorcontrib><creatorcontrib>Stevens, Marc J. A.</creatorcontrib><creatorcontrib>Cernela, Nicole</creatorcontrib><creatorcontrib>Zurfluh, Katrin</creatorcontrib><creatorcontrib>Muchaamba, Francis</creatorcontrib><creatorcontrib>Nüesch-Inderbinen, Magdalena</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raschle, Susanne</au><au>Stephan, Roger</au><au>Stevens, Marc J. A.</au><au>Cernela, Nicole</au><au>Zurfluh, Katrin</au><au>Muchaamba, Francis</au><au>Nüesch-Inderbinen, Magdalena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2021-04-27</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><spage>9066</spage><epage>9066</epage><pages>9066-9066</pages><artnum>9066</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Listeria monocytogenes
is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of
L. monocytogenes
to animals and the food chain. The present study assessed the occurrence of
L. monocytogenes
in 191 surface water samples from rivers, streams and inland canals throughout Switzerland. Twenty-five (13%) of the surface water samples contained
L. monocytogenes
. Whole genome sequence (WGS) data were used to characterize the 25 isolates. The isolates belonged to major lineages I and II, with the majority assigned to either serotype 1/2a (48%), or 4b (44%). The predominant CCs identified were the hypervirulent serotype 4b clones CC1 and CC4, and the serotype CC412; all three have been implicated in listeriosis outbreaks and sporadic cases of human and animal infection worldwide. Two (8%) of the isolates belonged to CC6 which is an emerging hypervirulent clone. All isolates contained intact genes associated with invasion and infection, including
inlA/B
and
prfA
. The four CC4 isolates all harbored
Listeria
pathogenicity island 4 (LIPI-4), which confers hypervirulence. The occurrence of
L. monocytogenes
in river ecosystems may contribute to the dissemination and introduction of clinically highly relevant strains to the food chain.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33907261</pmid><doi>10.1038/s41598-021-88514-y</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2045-2322 |
ispartof | Scientific reports, 2021-04, Vol.11 (1), p.9066-9066, Article 9066 |
issn | 2045-2322 2045-2322 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_edd7f122523f4b4999a486bace00a3b5 |
source | PMC (PubMed Central); Publicly Available Content (ProQuest); Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
subjects | 631/326 631/337 Animals Aquatic ecosystems Aquatic environment Bacterial Proteins - genetics Ecosystem Food chains Food irradiation Genetic Variation Genome, Bacterial Genomes Genomic Islands Genotype Humanities and Social Sciences Listeria Listeria monocytogenes Listeria monocytogenes - pathogenicity Listeriosis Listeriosis - genetics Listeriosis - microbiology multidisciplinary Nucleotide sequence Opportunist infection Pathogenicity Pathogens Rivers Science Science (multidisciplinary) Streams Surface water Switzerland Water analysis Water sampling Whole Genome Sequencing |
title | Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T03%3A05%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Environmental%20dissemination%20of%20pathogenic%20Listeria%20monocytogenes%20in%20flowing%20surface%20waters%20in%20Switzerland&rft.jtitle=Scientific%20reports&rft.au=Raschle,%20Susanne&rft.date=2021-04-27&rft.volume=11&rft.issue=1&rft.spage=9066&rft.epage=9066&rft.pages=9066-9066&rft.artnum=9066&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-021-88514-y&rft_dat=%3Cproquest_doaj_%3E2518853689%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c577t-2c424b94cff4673d4f638b83a37e415037d384df2d8a36049fb3a9ff917d4ae53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2518853689&rft_id=info:pmid/33907261&rfr_iscdi=true |