Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MSe

Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswe...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2019-12, Vol.9 (6), p.414-422
Main Authors: Katragunta, Kumar, Siva, Bandi, Kondepudi, Niharika, Vadaparthi, P.R. Rao, Rama Rao, Nadendla, Tiwari, Ashok Kumar, Suresh Babu, Katragadda
Format: Article
Language:English
Subjects:
Accuracy
Acids
Boswellia serrata
Boswellic acids
Calibration
Chromatography
Drug dosages
Herbal formulations
Isoforms
Laboratories
Metabolites
Methods
Original
Quantification
Secondary metabolites
Tirucallic acids
UPLC-Q-tof-MSe
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Summary:Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswellic acid (2), ɑ-Boswellic acid (3), β-Boswellic acid (4), 3-O-Acetyl-ɑ-boswellic acid (5) and 3-O-Acetyl-β-boswellic acid (6) in commercial herbal products containing B. serrata as an ingredient. Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B. serrata in rapid time using fragmentation approach than the traditional approaches. In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified. [Display omitted] •Development of UPLC-PDA method followed by Tof-MS/MS analysis for secondary metabolites characterization.•Quantification of six boswellic acids in crude extract & commercial herbal products available in the market.•Comprehensive analysis of crude extract using UPLC-Q-Tof-MSe.•Identification 16 secondary metabolites from Tof-MS/MS fragmentation pattern.
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2019.09.007