Porcine rotavirus C in pigs with gastroenteritis on Thai swine farms, 2011-2016

Swine are economically important food animals, but highly contagious porcine epidemic diarrhea virus (PEDV) and rotavirus can afflict pig herds and contribute significantly to piglet morbidity and mortality. While there have been studies on rotavirus group A (RVA) in Thailand, reports of rotavirus g...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2018-05, Vol.6, p.e4724-e4724, Article e4724
Main Authors: Tuanthap, Supansa, Phupolphan, Cherdpong, Luengyosluechakul, Supol, Duang-In, Ausanee, Theamboonlers, Apiradee, Wattanaphansak, Suphot, Vongpunsawad, Sompong, Amonsin, Alongkorn, Poovorawan, Yong
Format: Article
Language:English
Subjects:
Age
Analysis
Diarrhea
Disease
Diseases
Farms
Feces
Gastroenteritis
Genetic diversity
Genomes
Genotypes
Hogs
Infections
Intestine
Livestock farms
Molecular Biology
Morbidity
Mucosa
Phylogeny
Pigs
Polymerase chain reaction
Prevalence studies (Epidemiology)
Provinces
Rotavirus
Rotavirus C
Suidae
Swine
Swine diseases
Thailand
Transmissible gastroenteritis
Veterinary Medicine
Veterinary services
Viral infections
Virology
Viruses
VP4
VP4 gene
VP7
VP7 gene
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Summary:Swine are economically important food animals, but highly contagious porcine epidemic diarrhea virus (PEDV) and rotavirus can afflict pig herds and contribute significantly to piglet morbidity and mortality. While there have been studies on rotavirus group A (RVA) in Thailand, reports of rotavirus group C (RVC) are limited. Here, we aimed to identify the prevalence of RVC circulating on Thai commercial swine farms. We analyzed 769 feces and intestine mucosal contents of pigs affected with diarrhea between 2011 and 2016 using RT-PCR specific for the PEDV spike (S), rotavirus glycoprotein (G) VP7, and protease-sensitive protein (P) VP4 genes. We found that 6.6% (51/769) of samples tested positive for RVC, of which 11 samples were co-infected with RVA and four samples were co-infected with PEDV. Three samples tested positive for all three viruses. Phylogenetic analysis of the VP7 gene showed that the most frequent RVC genotype was G1, which grouped with the prototypic RVC Cowden strain. While G6 and G9 were also common, G3 was relatively rare. Analysis of the VP4 gene revealed that the most common P type was P[5], followed by P[4], P[7], and P[1]. In all, there were six G/P combinations (G6P[5], G1P[1], G1P[4], G1P[5], G9P[4], and G9P[7]), of which G6P[5] was the most predominant.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.4724