Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula

MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classif...

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Bibliographic Details
Published in:Genetics and molecular biology 2019-07, Vol.42 (3), p.611-623
Main Authors: Li, Wei, Liu, Ying, Zhao, Jinyue, Zhen, Xin, Guo, Changhong, Shu, Yongjun
Format: Article
Language:English
Subjects:
Abiotic stress
Alfalfa
BIOCHEMISTRY & MOLECULAR BIOLOGY
Chromosomes
Crosstalk
DNA microarrays
Functional analysis
Gene expression
Genes
GENETICS & HEREDITY
Genomes
Homology
Medicago truncatula
MYB
Nodulation
Phylogeny
Plant Genetics
qRT-PCR
Reproduction (copying)
Ribonucleic acid
RNA
Signal processing
Signal transduction
Stresses
Transcription factors
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Summary:MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the expression of MtMYB genes in response to abiotic stresses using RNA-seq data, which confirmed the critical roles in signal transduction and regulation processes under abiotic stress. We used quantitative real-time PCR (qRT-PCR) to validate expression profiles. The expression pattern of M. truncatula MYB genes under different abiotic stress conditions suggest that some may play a major role in cross-talk among different signal transduction pathways in response to abiotic stresses. Our study will serve as a foundation for future research into the molecular function of M. truncatula R2R3-MYB genes.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-gmb-2018-0235