Volatile Organic Compounds from Bacillus aryabhattai MCCC 1K02966 with Multiple Modes against Meloidogyne incognita

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-12, Vol.27 (1), p.103
• Main Authors: Chen, Wen, Wang, Jinping, Huang, Dian, Cheng, Wanli, Shao, Zongze, Cai, Minmin, Zheng, Longyu, Yu, Ziniu, Zhang, Jibin
• Format: Article
• Language: English
• Subjects: Agriculture; Animals; Antinematodal Agents - chemistry; Antinematodal Agents - pharmacology; Aquatic Organisms; Bacillus - metabolism; Bacillus aryabhattai; Biological control; Butanol; Crop production; Deep sea; Drug dosages; Drug resistance; Environmental conditions; Environmental protection; Fermentation; Gas chromatography; Gas Chromatography-Mass Spectrometry; Hatching; Mass spectrometry; Mass spectroscopy; Meloidogyne incognita; methyl thioacetate; Microorganisms; Mortality; multiple nematicidal modes; Nematodes; Organic compounds; Parasitic Sensitivity Tests; Pentane; Pesticides; Solid phases; Tylenchoidea - drug effects; VOCs; Volatile organic compounds; Volatile Organic Compounds - analysis; Volatile Organic Compounds - chemistry; Volatile Organic Compounds - pharmacology; Water Microbiology
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27010103

Plant-parasitic nematodes cause severe losses to crop production and economies all over the world. MCCC 1K02966, a deep-sea bacterium, was obtained from the Southwest Indian Ocean and showed nematicidal and fumigant activities against in vitro. The nematicidal volatile organic compounds (VOCs) from the fermentation broth of MCCC 1K02966 were investigated further using solid-phase microextraction gas chromatography-mass spectrometry. Four VOCs, namely, pentane, 1-butanol, methyl thioacetate, and dimethyl disulfide, were identified in the fermentation broth. Among these VOCs, methyl thioacetate exhibited multiple nematicidal activities, including contact nematicidal, fumigant, and repellent activities against . Methyl thioacetate showed a significant contact nematicidal activity with 87.90% mortality at 0.01 mg/mL by 72 h, fumigant activity in mortality 91.10% at 1 mg/mL by 48 h, and repellent activity at 0.01-10 mg/mL. In addition, methyl thioacetate exhibited 80-100% egg-hatching inhibition on the 7th day over the range of 0.5 mg/mL to 5 mg/mL. These results showed that methyl thioacetate from MCCC 1K02966 control with multiple nematicidal modes and can be used as a potential biological control agent.