Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS

• Published in: Mediators of Inflammation 2017-01, Vol.2017 (2017), p.1-14
• Main Authors: Su, Zi-Ren, Zhan, Janis Ya-xian, Zhou, Jiang-Tao, Huang, Yanfeng, Chen, Han-Bin, Wu, Qi-Duan, Zhang, Zhen-biao, Liu, Yu-Hong, Wu, Jia-Zhen, Liang, Jia-li, Dou, Yao-Xing
• Format: Article
• Language: English
• Subjects: Acetic acid; Animals; Anti-inflammatory agents; Biological activity; Carrageenan - toxicity; Cyclooxygenase 2 - metabolism; Cyclooxygenase-2; Cytokines; Cytosol; Dinoprostone - metabolism; Ear; Edema; Edema - chemically induced; Edema - drug therapy; Epoxy Compounds - chemistry; Epoxy Compounds - therapeutic use; Female; Gas Chromatography-Mass Spectrometry; Gene expression; Immunology; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; Interleukin 1; Interleukin 10; Interleukin 4; Interleukin 6; Interleukin-10 - metabolism; Interleukin-4 - metabolism; Interleukin-6 - metabolism; Male; Mass spectrometry; Medicine; Mice; mRNA; NF-kappa B - metabolism; NF-κB protein; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Nonsteroidal anti-inflammatory drugs; Oil; Permeability; Pharmaceutical sciences; Phosphorylation; Plant Oils - chemistry; Pogostemon - chemistry; Prostaglandin E2; Rodents; Scientific imaging; Tumor necrosis factor; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Vegetable oils; Xylene
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/2017/1089028

According to the GC-MS analysis, compositional variation was observed between samples of patchouli oil, of which an unknown compound identified as patchoulene epoxide (PAO) was found only in the long-stored oil, whose biological activity still remains unknown. Therefore, the present study aimed to evaluate the potential anti-inflammatory activity with three in vivo inflammatory models: xylene-induced ear edema, acetic acid-induced vascular permeability, and carrageenan-induced paw edema. Further investigation into its underlying mechanism on carrageenan-induced paw edema was conducted. Results demonstrated that PAO significantly inhibited the ear edema induced by xylene, lowered vascular permeability induced by acetic acid and decreased the paw edema induced by carrageenan. Moreover, PAO markedly decreased levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), prostaglandin E2 (PGE2), and nitric oxide (NO), but increased levels of interleukin-4 (IL-4) and interleukin-10 (IL-10). PAO was also shown to significantly downregulate the protein and mRNA expressions of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase (iNOS). Western blot analysis revealed that PAO remarkably inhibited p50 and p65 translocation from the cytosol to the nucleus by suppressing IKKβ and IκBα phosphorylation. In conclusion, PAO exhibited potent anti-inflammatory activity probably by suppressing the activation of iNOS, COX-2 and NF-κB signaling pathways.