Tumor targeting and imaging with dual-peptide conjugated multifunctional liposomal nanoparticles

• Published in: International journal of nanomedicine 2013-01, Vol.8 (1), p.4659-4671
• Main Authors: Rangger, Christine, Helbok, Anna, Sosabowski, Jane, Kremser, Christian, Koehler, Gottfried, Prassl, Ruth, Andreae, Fritz, Virgolini, Irene J, von Guggenberg, Elisabeth, Decristoforo, Clemens
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Cancer; Care and treatment; Cell Line; Drug targeting; dual-targeting; Female; Innovations; Lipids; liposomal nanoparticles; Liposomes - chemistry; Liposomes - pharmacokinetics; Medical imaging; Mice; Mice, Nude; Microscopy; Microscopy, Fluorescence; Molecular Imaging - methods; multifunctionality; Nanoparticles; Nanoparticles - chemistry; Nanotechnology; Neoplasms, Experimental - pathology; Neoplasms, Experimental - radiotherapy; NMR; Nuclear magnetic resonance; Original Research; Peptides; Peptides - chemistry; Peptides - pharmacokinetics; Pharmacology, Experimental; Polyethylene Glycols; radiolabeling; Radiopharmaceuticals - chemistry; Radiopharmaceuticals - pharmacokinetics; Tissue Distribution; Tomography; Tomography, Emission-Computed, Single-Photon - methods; Tomography, X-Ray Computed - methods; tumor imaging; Tumors; Xenograft Model Antitumor Assays
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S51927

The significant progress in nanotechnology provides a wide spectrum of nanosized material for various applications, including tumor targeting and molecular imaging. The aim of this study was to evaluate multifunctional liposomal nanoparticles for targeting approaches and detection of tumors using different imaging modalities. The concept of dual-targeting was tested in vitro and in vivo using liposomes derivatized with an arginine-glycine-aspartic acid (RGD) peptide binding to αvβ3 integrin receptors and a substance P peptide binding to neurokinin-1 receptors. For liposome preparation, lipids, polyethylene glycol building blocks, DTPA-derivatized lipids for radiolabeling, lipid-based RGD and substance P building blocks and imaging labels were combined in defined molar ratios. Liposomes were characterized by photon correlation spectroscopy and zeta potential measurements, and in vitro binding properties were tested using fluorescence microscopy. Standardized protocols for radiolabeling were developed to perform biodistribution and micro-single photon emission computed tomography/computed tomography (SPECT/CT) studies in nude mice bearing glioblastoma and/or melanoma tumor xenografts. Additionally, an initial magnetic resonance imaging study was performed. Liposomes were radiolabeled with high radiochemical yields. Fluorescence microscopy showed specific cellular interactions with RGD-liposomes and substance P-liposomes. Biodistribution and micro-SPECT/CT imaging of (111)In-labeled liposomal nanoparticles revealed low tumor uptake, but in a preliminary magnetic resonance imaging study with a single-targeted RGD-liposome, uptake in the tumor xenografts could be visualized. The present study shows the potential of liposomes as multifunctional targeted vehicles for imaging of tumors combining radioactive, fluorescent, and magnetic resonance signaling. Specific in vitro tumor targeting by fluorescence microscopy and radioactivity was achieved. However, biodistribution studies in an animal tumor model revealed only moderate tumor uptake and no additive effect using a dual-targeting approach.