Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil

Abstract INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of souther...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 2020-01, Vol.53
Main Authors: Mendes, Josiara Furtado, Poester, Vanice Rodrigues, Groll, Andrea Von, Meireles, Mário Carlos Araújo, Xavier, Melissa Orzechowski
Format: Article
Language:English
Subjects:
Environment
Paracoccidioides brasiliensis
Paracoccidioidomycosis
Short Communication
TROPICAL MEDICINE
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Summary:Abstract INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of southern Brazil. METHODS: Sixteen soil samples were submitted to nested-PCR and the amplicons of a representative number of positive samples were sequenced. RESULTS: Paracoccidioides spp. DNA was found in 44% of samples. Four DNA amplicons were sequenced, showing 100% homology with P. brasiliensis. CONCLUSIONS: The southern Brazilian urban population is commonly exposed to the Paracoccidioides fungus.
ISSN:0037-8682
1678-9849
1678-9849
DOI:10.1590/0037-8682-0172-2019