Transient Receptor Potential (TRP) Channels in the Pacific Oyster ( Crassostrea gigas ): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata

Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster ( ) and investigated their...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-03, Vol.22 (6), p.3222
Main Authors: Fu, Huiru, Jiao, Zexin, Li, Yongjing, Tian, Jing, Ren, Liting, Zhang, Fuqiang, Li, Qi, Liu, Shikai
Format: Article
Language:English
Subjects:
Animals
Computational Biology - methods
Crassostrea - classification
Crassostrea - physiology
Crassostrea gigas
Gene Dosage
gene expression
Gene Expression Profiling
Gene Expression Regulation
Genome-Wide Association Study
Heat-Shock Response - genetics
Invertebrates
Phenotype
Phylogeny
Stress, Physiological - genetics
thermal stress
Transcriptome
Transient Receptor Potential Channels - genetics
TRPs
Vertebrates
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Summary:Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster ( ) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the , which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between and , two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22063222