Overnight Corticosterone and Gene Expression in Mouse Hippocampus: Time Course during Resting Period

The aim of the experiment was to test the effect of an elevated level of glucocorticoids on the mouse hippocampal transcriptome after 12 h of treatment with corticosterone that was administered during an active phase of the circadian cycle. Additionally, we also tested the circadian changes in gene...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-02, Vol.24 (3), p.2828
Main Authors: Jaszczyk, Aneta, Stankiewicz, Adrian M, Goscik, Joanna, Majewska, Alicja, Jezierski, Tadeusz, Juszczak, Grzegorz R
Format: Article
Language:English
Subjects:
Animals
Aquaporin 4
brain
Cell culture
Circadian rhythms
Corticosterone
Corticosterone - metabolism
Corticosterone - pharmacology
Decay
Dimethylaniline monooxygenase (N-oxide-forming)
DNA microarrays
Gene expression
Gene Expression Profiling
Genes
Glucocorticoids
Glucocorticoids - metabolism
Hippocampus
Hippocampus - metabolism
Medical research
Metabolism
Mice
Physiology
Post traumatic stress disorder
Transcriptome
Transcriptomes
Transcriptomics
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Summary:The aim of the experiment was to test the effect of an elevated level of glucocorticoids on the mouse hippocampal transcriptome after 12 h of treatment with corticosterone that was administered during an active phase of the circadian cycle. Additionally, we also tested the circadian changes in gene expression and the decay time of transcriptomic response to corticosterone. Gene expression was analyzed using microarrays. Obtained results show that transcriptomic responses to glucocorticoids are heterogeneous in terms of the decay time with some genes displaying persistent changes in expression during 9 h of rest. We have also found a considerable overlap between genes regulated by corticosterone and genes implicated previously in stress response. The examples of such genes are , , , , , , , , , , , , , , , , , , , , , , , , , , , , , and . This indicates that the applied model is a useful tool for the investigation of mechanisms underlying the stress response.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032828