The use of platelet-rich plasma (PRP) to improve structural impairment of rat testis induced by busulfan

Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in infertile rat model by stereological method. Thirty-two male rats w...

Full description

Saved in:
Bibliographic Details
Published in:Platelets (Edinburgh) 2019-05, Vol.30 (4), p.513-520
Main Authors: Dehghani, Farzaneh, Sotoude, Narges, Bordbar, Hossein, Panjeshahin, M.R., Karbalay-Doust, Saied
Format: Article
Language:English
Subjects:
Busulfan
Platelet-rich plasma
stereology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in infertile rat model by stereological method. Thirty-two male rats were divided into four groups. Infertility was induced by the administration of busulfan (BUS) (10mg/kg, I.P., single dose). PRP (80μl, testis local injected, single dose) was administered for the subjects. After 48 days, semen analysis was performed and blood samples were taken from the heart to measure the testosterone. Then, the left testis was removed, fixed, embedded, sectioned, and stained by H after that the testes were analyzed. The results showed that BUS can decrease the sperm count, motility, normal morphology, length of the spermatozoon tail, volume of the testis, seminiferous tubules, germinal epithelium height, and the number of spermatogenesis lineage cells in comparison with the control group (p 
ISSN:0953-7104
1369-1635
DOI:10.1080/09537104.2018.1478400