Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time–tandem mass spectrometry

A novel method for the simultaneous determination of 3-nitrotyrosine(NT) and 3-chlorotyrosine(CT) in human plasma has been developed based on direct analysis in real time–tandem mass spectrometry(DART–MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring(M...

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Bibliographic Details
Published in:Acta pharmaceutica Sinica. B 2015-09, Vol.5 (5), p.482-486
Main Authors: Song, Yuqiao, Liao, Jie, Zha, Cheng, Wang, Bin, Liu, Charles C
Format: Article
Language:English
Subjects:
3-Chlorotyrosine
3-Nitrotyrosine
DART–MS/MS
Determintion
Human plasma
Short Communication
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Summary:A novel method for the simultaneous determination of 3-nitrotyrosine(NT) and 3-chlorotyrosine(CT) in human plasma has been developed based on direct analysis in real time–tandem mass spectrometry(DART–MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring(MRM) of the ion transitions at m/z 216.2/170.1 for CT, m/z 227.2/181.1 for NT and m/z 230.2/184.2 for the internal standard, d~3-NT. The assay was linear in the ranges 0.5–100 μg/m L for CT and 4–100 μg/m L for NT with corresponding limits of detection of 0.2 and 2 μg/m L. Intra- and inter-day precisions and accuracies were respectively <15% and ±15%. Matrix effects were also evaluated. The method is potentially useful for high throughput analysis although sensitivity needs to be improved before it can be applied in clinical research.
ISSN:2211-3835
2211-3843
DOI:10.1016/j.apsb.2015.07.004