Functional characterization of MdMYB73 reveals its involvement in cold stress response in apple calli and Arabidopsis

Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. F...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Integrative Agriculture 2017-10, Vol.16 (10), p.2215-2221
Main Authors: ZHANG, Quan-yan, YU, Jian-qiang, WANG, Jia-hui, HU, Da-gang, HAO, Yu-jin
Format: Article
Language:English
Subjects:
apple
CBF
cold stress
MdMYB73
MYB转录因子
soluble sugar
实时定量PCR
应激反应
愈伤组织
拟南芥
渗透调节物质
耐冷性
转基因苹果
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. Functional analysis showed that MdMYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR （qRT-PCR） assay indicated that the expression levels of cold-responsive genes including MdCBF2, MdCBF3, MdCBF4, and MdCBF5 were obviously enhanced in MdMYB73 transgenic calli, suggesting that MdMYB73 increased cold tolerance via C-repeat binding factor （CBF） cold response pathway. Finally, we found that soluble sugar, which provides an osmoticum for cells, was increased in MdMYB73 transgenic calli compared to that in the wild type control. These findings provide a new insights into how MdMYB73 is involved in cold stress response.
ISSN:2095-3119
2352-3425
DOI:10.1016/S2095-3119(17)61723-4