Posttranscriptional Regulation of RhBRC1 ( Rosa hybrida BRANCHED1 ) in Response to Sugars is Mediated via its Own 3' Untranslated Region, with a Potential Role of RhPUF4 (Pumilio RNA-Binding Protein Family)

• Published in: International journal of molecular sciences 2019-08, Vol.20 (15), p.3808
• Main Authors: Wang, Ming, Ogé, Laurent, Voisine, Linda, Perez-Garcia, Maria-Dolores, Jeauffre, Julien, Hibrand Saint-Oyant, Laurence, Grappin, Philippe, Hamama, Latifa, Sakr, Soulaiman
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; 3′UTR; Apical dominance; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Buds; Gene Expression Regulation, Plant - genetics; Genes; Genetics; Insects; Investigations; Life Sciences; Localization; Metabolism; mRNA; mRNA turnover; Phenotype; Plant breeding; Plant Proteins - genetics; Plants genetics; Polyadenylation; Post-transcription; Proteins; PUF protein; RhBRC1; RNA modification; RNA-binding protein; RNA-Binding Proteins - genetics; Rosa - genetics; Rosa - metabolism; shoot branching; Signal Transduction - genetics; Sorghum; Splicing; Stress response; Sucrose; sucrose metabolism; Sugar; Sugars - metabolism; Transcription Factors - genetics; Vegetal Biology; α-Amylase
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20153808

The shoot branching pattern is a determining phenotypic trait throughout plant development. During shoot branching, ( ) plays a master regulator role in bud outgrowth, and its transcript levels are regulated by various exogenous and endogenous factors. (the homologous gene of in ) is a main branching regulator whose posttranscriptional regulation in response to sugar was investigated through its 3'UTR. Transformed calluses containing a construction composed of the CaMV35S promoter, the green fluorescent protein (GFP) reporter gene, and the 3'UTR of (P35S:GFP::3'UTR ) were obtained and treated with various combinations of sugars and with sugar metabolism effectors. The results showed a major role of the 3'UTR of in response to sugars, involving glycolysis/the tricarboxylic acid cycle (TCA) and the oxidative pentose phosphate pathway (OPPP). In vegetative buds, sequence analysis of the 3'UTR identified six binding motifs specific to the Pumilio/FBF RNA-binding protein family (PUF) and probably involved in posttranscriptional regulation. was highly expressed in the buds of decapitated plants and in response to sugar availability in in-vitro-cultured buds. was found to be close to , which encodes an PUF protein. In addition, sugar-dependent upregulation of was also found in calluses. expression was especially dependent on the OPPP, supporting its role in OPPP-dependent posttranscriptional regulation of . These findings indicate that the 3'UTR sequence could be an important target in the molecular regulatory network of and pave the way for investigating new aspects of regulation.