cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi

Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia c...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2012-10, Vol.3 (4), p.1093-1104
Main Authors: Li, Fengjuan, Terenius, Olle, Li, Yuan, Fang, Suyun, Li, Wenli
Format: Article
Language:English
Subjects:
Antheraea pernyi
Bacillus subtilis
Baculoviridae
beta-glucans
complementary DNA
Escherichia coli
gene expression
genes
Gram-positive bacteria
GRP
hemocytes
immune response
Immunologi
Immunology
insect immunity
lectin
lectins
midgut
pupae
receptors
Saccharomyces cerevisiae
testes
tissues
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Summary:Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia coli, Antheraea pernyi nuclear polyhedrosis virus (ApNPV) and Saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbody, epidermis, testis, and hemocytes). ApβGRP and Aplectin-5 was induced by all the microorganisms, and mainly in epidermis and hemocytes, but not in testis; Aplectin-5 was also expressed in fatbody. Ap C-type lectin 1 was, on the contrary, highly expressed in testis and also in fatbody, but not in hemocytes. Unlike ApβGRP and Aplectin-5, Ap C-type lectin 1 was not induced by Gram-positive bacteria. The results suggest that the cloned lectins may have different functions in different tissues of A. pernyi.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects3041093