Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic Clostridioides difficile Colonization and Infection

In this study, we aimed to evaluate the composition of the intestinal microbiota and level of fecal calprotectin in Clostridioides difficile-colonized patients. We included 102 C. difficile non-colonized (group I), 93 C. difficile colonized subjects (group II), and 89 diarrhea patients with C. diffi...

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Bibliographic Details
Published in:Microorganisms (Basel) 2020-06, Vol.8 (6), p.882
Main Authors: Han, Sung-Hee, Yi, Joowon, Kim, Ji-Hoon, Moon, Hee-Won
Format: Article
Language:English
Subjects:
Antibiotics
calprotectin
Clostridioides difficile infection
Clostridium
Colonization
Deoxyribonucleic acid
Diarrhea
DNA
Feces
Genes
Health aspects
Host-bacteria relationships
Infections
Inflammation
Intestinal microflora
Intestine
Levels
Microbiota
Observations
Relative abundance
Software
Statistical analysis
Variance analysis
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Summary:In this study, we aimed to evaluate the composition of the intestinal microbiota and level of fecal calprotectin in Clostridioides difficile-colonized patients. We included 102 C. difficile non-colonized (group I), 93 C. difficile colonized subjects (group II), and 89 diarrhea patients with C. difficile (group III). Chao1 index for alpha diversity and principal coordinate analysis was performed for beta diversity using QIIME. The mean relative abundance in each group was compared at the phylum and genus levels. Fecal calprotectin was measured using EliA calprotectin (Thermo Fisher Scientific). Group II showed significantly lower levels of Sutterella, Blautia, Ruminococcus, Faecalibacterium, Bilophila, and Ruminococcaceae and higher levels of Enterobacteriaceae compared to group I (p = 0.012, 0.003, 0.002, 0.001, 0.027, 0.022, and 0.036, respectively). Toxigenic C. difficile colonized subjects showed significantly lower levels of Prevotella, Phascolarctobacterium, Succinivibrio, Blautia, and higher levels of Bacteroides. The level of fecal calprotectin in group III was significantly higher than those in group I and group II (p < 0.001 for both). These data could be valuable in understanding C. difficile colonization process and the microbiota and inflammatory markers could be further studied to differentiate colonization from CDI.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8060882