A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay

Point-of-care (PoC) devices offer a promising solution for fast, portable, and easy-to-use diagnostics. These characteristics are particularly relevant in agrifood fields like viticulture where the early detection of plant stresses is crucial to crop yield. Microfluidics, with its low reagent volume...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2025-01, Vol.25 (2), p.411
Main Authors: Domingues, Cristiana, Rodrigues, Marta S C, Condelipes, Pedro G M, Fortes, Ana Margarida, Chu, Virginia, Conde, João Pedro
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic Acid - analysis
Abscisic Acid - metabolism
Aluminum
Biomarkers
capillarity
Contact angle
COVID-19
Crop diseases
Design
Dimethylpolysiloxanes - chemistry
hydrophilic polydimethylsiloxane copolymer (PDMS-PEG)
Immunoassay
Immunoassay - instrumentation
Immunoassay - methods
Infections
Lab-On-A-Chip Devices
Laboratory equipment
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
microfluidics
Microfluidics - instrumentation
Microfluidics - methods
Polyethylene glycol
Polyethylene Glycols - chemistry
Porous materials
portability
Reagents
Sensors
Silicon wafers
Vitis - physiology
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Summary:Point-of-care (PoC) devices offer a promising solution for fast, portable, and easy-to-use diagnostics. These characteristics are particularly relevant in agrifood fields like viticulture where the early detection of plant stresses is crucial to crop yield. Microfluidics, with its low reagent volume requirements, is well-suited for such applications. Self-driven microfluidic devices, which rely on capillary forces for fluid motion, offer an attractive alternative by eliminating the need for external pumps and complex fluid control systems. However, traditional microfluidic prototyping materials like polydimethylsiloxane (PDMS) present challenges due to their hydrophobic nature. This paper presents the development of a reusable, portable, capillary-driven microfluidic platform based on a PDMS-PEG (polyethylene glycol) copolymer designed for the rapid low-cost detection of abscisic acid (ABA), a key biomarker for the onset of ripening of non-climacteric fruits and drought stress in vines. By employing passive fluid transport mechanisms, such as capillary-driven sequential flow, this platform enables precise biological and chemical screenings while maintaining portability and ease of use. A simplified field-ready sample processing method is used to prepare the grapes for analysis.
ISSN:1424-8220
1424-8220
DOI:10.3390/s25020411