Effect of HIT Components on the Development of Breast Cancer Cells

Cancer cells circulating in blood vessels activate platelets, forming a cancer cell encircling platelet cloak which facilitates cancer metastasis. Heparin (H) is frequently used as an anticoagulant in cancer patients but up to 5% of patients have a side effect, heparin-induced thrombocytopenia (HIT)...

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Bibliographic Details
Published in:Life (Basel, Switzerland) Switzerland), 2021-08, Vol.11 (8), p.832
Main Authors: Chen, Li-Yu, Apte, Gurunath, Lindenbauer, Annerose, Frant, Marion, Nguyen, Thi-Huong
Format: Article
Language:English
Subjects:
Angiogenesis
Antibodies
Atomic force microscopy
Binding sites
Blood circulation
Blood platelets
Blood vessels
Breast cancer
breast cancer cells
Cell culture
Cell growth
Cell proliferation
Cell spreading
Confocal microscopy
Coronaviruses
COVID-19
Growth factors
Heparin
Mechanical properties
Metastases
Metastasis
Microscopy
monoclonal antibodies
Platelet factor 4
Platelets
Polyethylene glycol
Scanning microscopy
Thrombocytopenia
Thrombosis
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Summary:Cancer cells circulating in blood vessels activate platelets, forming a cancer cell encircling platelet cloak which facilitates cancer metastasis. Heparin (H) is frequently used as an anticoagulant in cancer patients but up to 5% of patients have a side effect, heparin-induced thrombocytopenia (HIT) that can be life-threatening. HIT is developed due to a complex interaction among multiple components including heparin, platelet factor 4 (PF4), HIT antibodies, and platelets. However, available information regarding the effect of HIT components on cancers is limited. Here, we investigated the effect of these materials on the mechanical property of breast cancer cells using atomic force microscopy (AFM) while cell spreading was quantified by confocal laser scanning microscopy (CLSM), and cell proliferation rate was determined. Over time, we found a clear effect of each component on cell elasticity and cell spreading. In the absence of platelets, HIT antibodies inhibited cell proliferation but they promoted cell proliferation in the presence of platelets. Our results indicate that HIT complexes influenced the development of breast cancer cells.
ISSN:2075-1729
2075-1729
DOI:10.3390/life11080832