Soil enzyme activities and microbial community modulation after addition of poultry litter amendment enriched with Bacillus spp

• Published in: Ciência e agrotecnologia 2023-01, Vol.47
• Main Authors: Alves, Leonardo Cardoso, Silva, João Fernando Marques da, Tornisiello, Danilo Cunha, Betioli Junior, Edner, Andreote, Fernando Dini, Pauli, Giuliano
• Format: Article
• Language: English
• Subjects: AGRICULTURE, MULTIDISCIPLINARY; alpha diversity; Ecology; organic matter; soil bacterial community; sustainable agricultural practices
• ISSN: 1413-7054; 1981-1829; 1981-1829
• DOI: 10.1590/1413-7054202347006223

ABSTRACT The global consumption of mineral fertilizers has increased in the last 60 years. However, these fertilizers can negatively affect the soil and the atmosphere. The application of soil amendments focusing on sustainable farming practices can reduce the effects of mineral fertilization. In this study, we investigated the effects of increasing the dose of a commercial amendment using poultry litter enriched with Bacillus (SMARTGRAN) in different types of soils in Brazil under microcosm conditions. These different types of soils were classified as Nitossolo, Argissolo arênico, Argissolo alumínico, Latossolo distroférrico, and Latossolo distrófico. The biological activity in the soil was quantified by measuring the enzymes arylsulfatase, beta-glucosidase, and acid phosphatase. Additionally, bacterial diversity was evaluated by amplicon sequencing of the 16S rRNA gene and conducting phylogenetic analyses of three types of soils, which were selected because of their occurrence and fertility profiles. The results showed an increase in those enzyme activities under all conditions. The results of the analysis of the bacterial community in Nitossolo, Argissolo arênico, and Latossolo distrófico soil types showed a direct relationship between the bacterial composition in the soil and the increase in the amendment dosage. The alpha diversity indices decreased considerably because some plant-growth-promoting bacteria, such as Bacillus, Massilia, Paenibacillus, and Rhizobium, increased in relative abundance. The results indicated that an organic amendment enriched with Bacillus had a beneficial effect on different types of soil in Brazil. RESUMO Nos últimos 60 anos o consumo global de fertilizantes aumentou, porém estes produtos podem causar efeitos negativos ao solo e atmosfera. A aplicação de condicionadores de solos visando práticas sustentáveis pode reduzir os impactos da fertilização mineral. No presente estudo, os efeitos de doses crescentes de um condicionador comercial feito a partir de cama de frango enriquecida com Bacillus (SMARTGRAN) foram investigados em microcosmo para diferentes tipos de solos brasileiros. Os solos foram classificados como Nitossolo, Argissolo arênico, Argissolo alumínico, Latossolo distroférrico e Latossolo distrófico. A atividade biológica foi quantificada através da medição das enzimas arilsulfatase, beta-glicosidase e fosfatase ácida. Além disso, a diversidade de bactérias foi avaliada com sequenciamento de amplicons de gene pa