Loading…

Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells

Peste des petits ruminants virus (PPRV) is a highly contagious pathogen that strongly influences the productivity of small ruminants worldwide. Acetylation is an important post-translational modification involved in regulation of multiple biological functions. However, the extent and function of ace...

Full description

Saved in:
Bibliographic Details
Published in:Virology journal 2023-10, Vol.20 (1), p.1-227, Article 227
Main Authors: Meng, Xuelian, Wang, Xiangwei, Zhu, Xueliang, Zhang, Rui, Zhang, Zhidong, Sun, Yuefeng
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c526t-290c49d50150fd37584ab26a795cd7d4c641b23299214e41f044f71184792ee03
container_end_page 227
container_issue 1
container_start_page 1
container_title Virology journal
container_volume 20
creator Meng, Xuelian
Wang, Xiangwei
Zhu, Xueliang
Zhang, Rui
Zhang, Zhidong
Sun, Yuefeng
description Peste des petits ruminants virus (PPRV) is a highly contagious pathogen that strongly influences the productivity of small ruminants worldwide. Acetylation is an important post-translational modification involved in regulation of multiple biological functions. However, the extent and function of acetylation in host cells during PPRV infection remains unknown. Dimethylation-labeling-based quantitative proteomic analysis of the acetylome of PPRV-infected Vero cells was performed. In total, 1068 proteins with 2641 modification sites were detected in response to PPRV infection, of which 304 differentially acetylated proteins (DAcPs) with 410 acetylated sites were identified (fold change < 0.83 or > 1.2 and P < 0.05), including 109 up-regulated and 195 down-regulated proteins. Gene Ontology (GO) classification indicated that DAcPs were mostly located in the cytoplasm (43%) and participated in cellular and metabolic processes related to binding and catalytic activity. Functional enrichment indicated that the DAcPs were involved in the minichromosome maintenance complex, unfolded protein binding, helicase activity. Only protein processing in endoplasmic reticulum pathway was enriched. A protein-protein interaction (PPI) network of the identified proteins further indicated that a various chaperone and ribosome processes were modulated by acetylation. To the best of our knowledge, this is the first study on acetylome in PPRV-infected host cell. Our findings establish an important baseline for future study on the roles of acetylation in the host response to PPRV replication and provide novel insights for understanding the molecular pathological mechanism of PPRV infection.
doi_str_mv 10.1186/s12985-023-02200-1
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_ef7cf56f2fd949b8ac3fbadb7bffce97</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A768461970</galeid><doaj_id>oai_doaj_org_article_ef7cf56f2fd949b8ac3fbadb7bffce97</doaj_id><sourcerecordid>A768461970</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-290c49d50150fd37584ab26a795cd7d4c641b23299214e41f044f71184792ee03</originalsourceid><addsrcrecordid>eNptkk2LFDEQhhtRcF39A54CXvTQu0k66XROsiyrDiwsfuJBCOmkMmbo7oxJenD-vZmZRW2REFJUnnpDvamqek7wBSFde5kIlR2vMW3KphjX5EF1RgRrakbp14d_xY-rJyltMG5oK-RZ9e39rKfss85-B0hPetgnn1BwSBvI-6Hkw4T8hLaQMiALqUSFTyjOo59KbUI7H-dU-8mByWDRF4gBGRiG9LR65PSQ4Nn9eV59fnPz6fpdfXv3dnV9dVsbTttcU4kNk5ZjwrGzjeAd0z1ttZDcWGGZaRnpaUOlpIQBIw4z5kTpmwlJAXBzXq1OujbojdpGP-q4V0F7dUyEuFY6Zm8GUOCEcbx11FnJZN9p07he2170zhmQomi9Pmlt534Ea2DKUQ8L0eXN5L-rddgpgnnbUMKLwst7hRh-zMU2Nfp08ENPEOakaFc65KTsgr74B92EOZZPOFKCY865-EOtdemg-BzKw-Ygqq5E27GWSHEw4eI_VFkWRm_CBM6X_KLg1aKgMBl-5rWeU1Krjx-WLD2xJoaUIrjfhhCsDhOoThOoygSq4wQq0vwC_F_N9g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2877505557</pqid></control><display><type>article</type><title>Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells</title><source>Open Access: PubMed Central</source><source>Publicly Available Content (ProQuest)</source><source>Coronavirus Research Database</source><creator>Meng, Xuelian ; Wang, Xiangwei ; Zhu, Xueliang ; Zhang, Rui ; Zhang, Zhidong ; Sun, Yuefeng</creator><creatorcontrib>Meng, Xuelian ; Wang, Xiangwei ; Zhu, Xueliang ; Zhang, Rui ; Zhang, Zhidong ; Sun, Yuefeng</creatorcontrib><description>Peste des petits ruminants virus (PPRV) is a highly contagious pathogen that strongly influences the productivity of small ruminants worldwide. Acetylation is an important post-translational modification involved in regulation of multiple biological functions. However, the extent and function of acetylation in host cells during PPRV infection remains unknown. Dimethylation-labeling-based quantitative proteomic analysis of the acetylome of PPRV-infected Vero cells was performed. In total, 1068 proteins with 2641 modification sites were detected in response to PPRV infection, of which 304 differentially acetylated proteins (DAcPs) with 410 acetylated sites were identified (fold change &lt; 0.83 or &gt; 1.2 and P &lt; 0.05), including 109 up-regulated and 195 down-regulated proteins. Gene Ontology (GO) classification indicated that DAcPs were mostly located in the cytoplasm (43%) and participated in cellular and metabolic processes related to binding and catalytic activity. Functional enrichment indicated that the DAcPs were involved in the minichromosome maintenance complex, unfolded protein binding, helicase activity. Only protein processing in endoplasmic reticulum pathway was enriched. A protein-protein interaction (PPI) network of the identified proteins further indicated that a various chaperone and ribosome processes were modulated by acetylation. To the best of our knowledge, this is the first study on acetylome in PPRV-infected host cell. Our findings establish an important baseline for future study on the roles of acetylation in the host response to PPRV replication and provide novel insights for understanding the molecular pathological mechanism of PPRV infection.</description><identifier>ISSN: 1743-422X</identifier><identifier>EISSN: 1743-422X</identifier><identifier>DOI: 10.1186/s12985-023-02200-1</identifier><language>eng</language><publisher>London: BioMed Central Ltd</publisher><subject>Acetylation ; Amino acids ; Antibodies ; Care and treatment ; Cytoplasm ; Diseases ; DNA helicase ; Endoplasmic reticulum ; Enzymes ; Ethylenediaminetetraacetic acid ; Health aspects ; Infection ; Infections ; Methylation ; Pathogenesis ; Peptides ; Peste des petits ruminants ; Peste des petits ruminants virus ; Physiological aspects ; Post-translation ; Post-translational modification ; Post-translational modification, protein-protein interaction ; Protein binding ; Protein folding ; Protein interaction ; Protein-protein interactions ; Proteins ; Proteomics ; Quantitative analysis ; Ruminants ; Vero cells ; Viral infections ; Viruses</subject><ispartof>Virology journal, 2023-10, Vol.20 (1), p.1-227, Article 227</ispartof><rights>COPYRIGHT 2023 BioMed Central Ltd.</rights><rights>2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>BioMed Central Ltd., part of Springer Nature 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c526t-290c49d50150fd37584ab26a795cd7d4c641b23299214e41f044f71184792ee03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563215/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2877505557?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,38516,43895,44590,53791,53793</link.rule.ids></links><search><creatorcontrib>Meng, Xuelian</creatorcontrib><creatorcontrib>Wang, Xiangwei</creatorcontrib><creatorcontrib>Zhu, Xueliang</creatorcontrib><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Zhang, Zhidong</creatorcontrib><creatorcontrib>Sun, Yuefeng</creatorcontrib><title>Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells</title><title>Virology journal</title><description>Peste des petits ruminants virus (PPRV) is a highly contagious pathogen that strongly influences the productivity of small ruminants worldwide. Acetylation is an important post-translational modification involved in regulation of multiple biological functions. However, the extent and function of acetylation in host cells during PPRV infection remains unknown. Dimethylation-labeling-based quantitative proteomic analysis of the acetylome of PPRV-infected Vero cells was performed. In total, 1068 proteins with 2641 modification sites were detected in response to PPRV infection, of which 304 differentially acetylated proteins (DAcPs) with 410 acetylated sites were identified (fold change &lt; 0.83 or &gt; 1.2 and P &lt; 0.05), including 109 up-regulated and 195 down-regulated proteins. Gene Ontology (GO) classification indicated that DAcPs were mostly located in the cytoplasm (43%) and participated in cellular and metabolic processes related to binding and catalytic activity. Functional enrichment indicated that the DAcPs were involved in the minichromosome maintenance complex, unfolded protein binding, helicase activity. Only protein processing in endoplasmic reticulum pathway was enriched. A protein-protein interaction (PPI) network of the identified proteins further indicated that a various chaperone and ribosome processes were modulated by acetylation. To the best of our knowledge, this is the first study on acetylome in PPRV-infected host cell. Our findings establish an important baseline for future study on the roles of acetylation in the host response to PPRV replication and provide novel insights for understanding the molecular pathological mechanism of PPRV infection.</description><subject>Acetylation</subject><subject>Amino acids</subject><subject>Antibodies</subject><subject>Care and treatment</subject><subject>Cytoplasm</subject><subject>Diseases</subject><subject>DNA helicase</subject><subject>Endoplasmic reticulum</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Health aspects</subject><subject>Infection</subject><subject>Infections</subject><subject>Methylation</subject><subject>Pathogenesis</subject><subject>Peptides</subject><subject>Peste des petits ruminants</subject><subject>Peste des petits ruminants virus</subject><subject>Physiological aspects</subject><subject>Post-translation</subject><subject>Post-translational modification</subject><subject>Post-translational modification, protein-protein interaction</subject><subject>Protein binding</subject><subject>Protein folding</subject><subject>Protein interaction</subject><subject>Protein-protein interactions</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Quantitative analysis</subject><subject>Ruminants</subject><subject>Vero cells</subject><subject>Viral infections</subject><subject>Viruses</subject><issn>1743-422X</issn><issn>1743-422X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>COVID</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkk2LFDEQhhtRcF39A54CXvTQu0k66XROsiyrDiwsfuJBCOmkMmbo7oxJenD-vZmZRW2REFJUnnpDvamqek7wBSFde5kIlR2vMW3KphjX5EF1RgRrakbp14d_xY-rJyltMG5oK-RZ9e39rKfss85-B0hPetgnn1BwSBvI-6Hkw4T8hLaQMiALqUSFTyjOo59KbUI7H-dU-8mByWDRF4gBGRiG9LR65PSQ4Nn9eV59fnPz6fpdfXv3dnV9dVsbTttcU4kNk5ZjwrGzjeAd0z1ttZDcWGGZaRnpaUOlpIQBIw4z5kTpmwlJAXBzXq1OujbojdpGP-q4V0F7dUyEuFY6Zm8GUOCEcbx11FnJZN9p07he2170zhmQomi9Pmlt534Ea2DKUQ8L0eXN5L-rddgpgnnbUMKLwst7hRh-zMU2Nfp08ENPEOakaFc65KTsgr74B92EOZZPOFKCY865-EOtdemg-BzKw-Ygqq5E27GWSHEw4eI_VFkWRm_CBM6X_KLg1aKgMBl-5rWeU1Krjx-WLD2xJoaUIrjfhhCsDhOoThOoygSq4wQq0vwC_F_N9g</recordid><startdate>20231010</startdate><enddate>20231010</enddate><creator>Meng, Xuelian</creator><creator>Wang, Xiangwei</creator><creator>Zhu, Xueliang</creator><creator>Zhang, Rui</creator><creator>Zhang, Zhidong</creator><creator>Sun, Yuefeng</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20231010</creationdate><title>Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells</title><author>Meng, Xuelian ; Wang, Xiangwei ; Zhu, Xueliang ; Zhang, Rui ; Zhang, Zhidong ; Sun, Yuefeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-290c49d50150fd37584ab26a795cd7d4c641b23299214e41f044f71184792ee03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acetylation</topic><topic>Amino acids</topic><topic>Antibodies</topic><topic>Care and treatment</topic><topic>Cytoplasm</topic><topic>Diseases</topic><topic>DNA helicase</topic><topic>Endoplasmic reticulum</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Health aspects</topic><topic>Infection</topic><topic>Infections</topic><topic>Methylation</topic><topic>Pathogenesis</topic><topic>Peptides</topic><topic>Peste des petits ruminants</topic><topic>Peste des petits ruminants virus</topic><topic>Physiological aspects</topic><topic>Post-translation</topic><topic>Post-translational modification</topic><topic>Post-translational modification, protein-protein interaction</topic><topic>Protein binding</topic><topic>Protein folding</topic><topic>Protein interaction</topic><topic>Protein-protein interactions</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Quantitative analysis</topic><topic>Ruminants</topic><topic>Vero cells</topic><topic>Viral infections</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meng, Xuelian</creatorcontrib><creatorcontrib>Wang, Xiangwei</creatorcontrib><creatorcontrib>Zhu, Xueliang</creatorcontrib><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Zhang, Zhidong</creatorcontrib><creatorcontrib>Sun, Yuefeng</creatorcontrib><collection>CrossRef</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Virology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meng, Xuelian</au><au>Wang, Xiangwei</au><au>Zhu, Xueliang</au><au>Zhang, Rui</au><au>Zhang, Zhidong</au><au>Sun, Yuefeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells</atitle><jtitle>Virology journal</jtitle><date>2023-10-10</date><risdate>2023</risdate><volume>20</volume><issue>1</issue><spage>1</spage><epage>227</epage><pages>1-227</pages><artnum>227</artnum><issn>1743-422X</issn><eissn>1743-422X</eissn><abstract>Peste des petits ruminants virus (PPRV) is a highly contagious pathogen that strongly influences the productivity of small ruminants worldwide. Acetylation is an important post-translational modification involved in regulation of multiple biological functions. However, the extent and function of acetylation in host cells during PPRV infection remains unknown. Dimethylation-labeling-based quantitative proteomic analysis of the acetylome of PPRV-infected Vero cells was performed. In total, 1068 proteins with 2641 modification sites were detected in response to PPRV infection, of which 304 differentially acetylated proteins (DAcPs) with 410 acetylated sites were identified (fold change &lt; 0.83 or &gt; 1.2 and P &lt; 0.05), including 109 up-regulated and 195 down-regulated proteins. Gene Ontology (GO) classification indicated that DAcPs were mostly located in the cytoplasm (43%) and participated in cellular and metabolic processes related to binding and catalytic activity. Functional enrichment indicated that the DAcPs were involved in the minichromosome maintenance complex, unfolded protein binding, helicase activity. Only protein processing in endoplasmic reticulum pathway was enriched. A protein-protein interaction (PPI) network of the identified proteins further indicated that a various chaperone and ribosome processes were modulated by acetylation. To the best of our knowledge, this is the first study on acetylome in PPRV-infected host cell. Our findings establish an important baseline for future study on the roles of acetylation in the host response to PPRV replication and provide novel insights for understanding the molecular pathological mechanism of PPRV infection.</abstract><cop>London</cop><pub>BioMed Central Ltd</pub><doi>10.1186/s12985-023-02200-1</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1743-422X
ispartof Virology journal, 2023-10, Vol.20 (1), p.1-227, Article 227
issn 1743-422X
1743-422X
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_ef7cf56f2fd949b8ac3fbadb7bffce97
source Open Access: PubMed Central; Publicly Available Content (ProQuest); Coronavirus Research Database
subjects Acetylation
Amino acids
Antibodies
Care and treatment
Cytoplasm
Diseases
DNA helicase
Endoplasmic reticulum
Enzymes
Ethylenediaminetetraacetic acid
Health aspects
Infection
Infections
Methylation
Pathogenesis
Peptides
Peste des petits ruminants
Peste des petits ruminants virus
Physiological aspects
Post-translation
Post-translational modification
Post-translational modification, protein-protein interaction
Protein binding
Protein folding
Protein interaction
Protein-protein interactions
Proteins
Proteomics
Quantitative analysis
Ruminants
Vero cells
Viral infections
Viruses
title Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A19%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20analysis%20of%20acetylation%20in%20peste%20des%20petits%20ruminants%20virus-infected%20Vero%20cells&rft.jtitle=Virology%20journal&rft.au=Meng,%20Xuelian&rft.date=2023-10-10&rft.volume=20&rft.issue=1&rft.spage=1&rft.epage=227&rft.pages=1-227&rft.artnum=227&rft.issn=1743-422X&rft.eissn=1743-422X&rft_id=info:doi/10.1186/s12985-023-02200-1&rft_dat=%3Cgale_doaj_%3EA768461970%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c526t-290c49d50150fd37584ab26a795cd7d4c641b23299214e41f044f71184792ee03%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2877505557&rft_id=info:pmid/&rft_galeid=A768461970&rfr_iscdi=true