Rebalancing Redox to Improve Biobutanol Production by Clostridium tyrobutyricum

Biobutanol is a sustainable green biofuel that can substitute for gasoline. Carbon flux has been redistributed in via metabolic cell engineering to produce biobutanol. However, the lack of reducing power hampered the further improvement of butanol production. The objective of this study was to impro...

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Bibliographic Details
Published in:Bioengineering (Basel) 2015-12, Vol.3 (1), p.2
Main Authors: Ma, Chao, Ou, Jianfa, Xu, Ningning, Fierst, Janna L, Yang, Shang-Tian, Liu, Xiaoguang
Format: Article
Language:English
Subjects:
Alcohol
Alcohol dehydrogenase
Bacteria
Bioengineering
Bioreactors
Butanol
butanol production
Carbon
Clostridium
Clostridium tyrobutyricum
Cyanocobalamin
Dehydrogenases
E coli
Engineering
Ethanol
Fermentation
Formate dehydrogenase
Gasoline
Gene expression
metabolic cell engineering
Metabolic engineering
Metabolic flux
metabolic shift
Metabolism
Methyl viologen
NADH
Nicotinamide adenine dinucleotide
Pneumonia
Productivity
redox engineering
Sodium
Sodium formate
Vanadate
Vitamin B12
Yeast
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Summary:Biobutanol is a sustainable green biofuel that can substitute for gasoline. Carbon flux has been redistributed in via metabolic cell engineering to produce biobutanol. However, the lack of reducing power hampered the further improvement of butanol production. The objective of this study was to improve butanol production by rebalancing redox. Firstly, a metabolically-engineered mutant CTC- - was constructed by introducing heterologous formate dehydrogenase ( ) and bifunctional aldehyde/alcohol dehydrogenase ( ) simultaneously into wild-type . The mutant evaluation indicated that the -catalyzed NADH-producing pathway improved butanol titer by 2.15-fold in the serum bottle and 2.72-fold in the bioreactor. Secondly, the medium supplements that could shift metabolic flux to improve the production of butyrate or butanol were identified, including vanadate, acetamide, sodium formate, vitamin B12 and methyl viologen hydrate. Finally, the free-cell fermentation produced 12.34 g/L of butanol from glucose using the mutant CTC- - , which was 3.88-fold higher than that produced by the control mutant CTC- . This study demonstrated that the redox engineering in could greatly increase butanol production.
ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering3010002