Transfer of a mobile Staphylococcus saprophyticus plasmid isolated from fermented seafood that confers tetracycline resistance

The complete nucleotide sequence of a tetracycline-resistance gene (tetK)-carrying plasmid from a Staphylococcus saprophyticus isolate from jeotgal, a Korean high-salt-fermented seafood, was determined. The plasmid, designated pSSTET1, was 4439 bp in length and encoded typical elements found in plas...

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Bibliographic Details
Published in:PloS one 2019-02, Vol.14 (2), p.e0213289-e0213289
Main Authors: Lee, Jong-Hoon, Heo, Sojeong, Jeong, Miran, Jeong, Do-Won
Format: Article
Language:English
Subjects:
Animals
Base sequence
Biology and Life Sciences
Conjugation, Genetic
Dosage and administration
Enterococcus faecalis - drug effects
Enterococcus faecalis - genetics
Enzymes
Fermented Foods and Beverages - microbiology
Food Microbiology
Genes
Genes, Bacterial
Humans
Medicine and Health Sciences
Microbial drug resistance
Penicillin G
Plasmids
Republic of Korea
Research and Analysis Methods
RNA
Seafood - microbiology
Staphylococcus saprophyticus - drug effects
Staphylococcus saprophyticus - genetics
Tetracycline Resistance - genetics
Tetracyclines
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Summary:The complete nucleotide sequence of a tetracycline-resistance gene (tetK)-carrying plasmid from a Staphylococcus saprophyticus isolate from jeotgal, a Korean high-salt-fermented seafood, was determined. The plasmid, designated pSSTET1, was 4439 bp in length and encoded typical elements found in plasmids that replicate via a rolling-circle mechanism, including the replication protein gene (rep), a double-stranded origin of replication, a single-stranded origin of replication, and a counter-transcribed RNA sequence. Additionally, the plasmid recombination enzyme gene (pre), which may be involved in inter-plasmid recombination and conjugation, was found. Each gene exhibited >94% sequence identity with those harbored in other Staphylococcus species. pSSTET1 was conditionally transferred to Staphylococcus species in a host-dependent manner and transferred to an Enterococcus faecalis strain in vitro. Antibiotic susceptibility of the transconjugants was host-dependent and transconjugants maintained a tetracycline-resistant phenotype in the absence of selective pressure over 100 generations.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0213289