Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage

The platelet-rich fibrin-like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (W...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2017-09, Vol.5 (3), p.57
Main Authors: Kawabata, Hideo, Isobe, Kazushige, Watanabe, Taisuke, Okudera, Toshimitsu, Nakamura, Masayuki, Suzuki, Masashi, Ryu, Jietsu, Kitamura, Yutaka, Okudera, Hajime, Okuda, Kazuhiro, Nakata, Koh, Kawase, Tomoyuki
Format: Article
Language:English
Subjects:
Blood
Blood cells
Blood platelets
Calcium chloride
Citric acid
Dextrose
Electron microscopy
Enzyme-linked immunosorbent assay
Fibers
Fibrin
fibrin fiber
Flow cytometry
Platelet-derived growth factor
Platelet-derived growth factor BB
platelet-rich fibrin
Platelets
Quality control
Scanning electron microscopy
Storage
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The platelet-rich fibrin-like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaClâ‚‚ in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared-without evident loss of quality-from WB samples stored for up to 7 days by our previously developed method.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines5030057