Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient

Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emissio...

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Bibliographic Details
Published in:Stem cell research 2016-03, Vol.16 (2), p.213-215
Main Authors: Lee, Han-Kyu, Morin, Peter, Xia, Weiming
Format: Article
Language:English
Subjects:
Alzheimer Disease - diagnostic imaging
Alzheimer Disease - metabolism
Alzheimer Disease - pathology
Cell Differentiation
Cellular Reprogramming
Embryoid Bodies - cytology
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Leukocytes, Mononuclear - cytology
Male
Microscopy, Fluorescence
Middle Aged
Positron-Emission Tomography
Sendai virus
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus. Three germ layer differentiation was induced to demonstrate the pluripotency of these iPSCs.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2015.12.050