Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We...

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Bibliographic Details
Published in:Viruses 2023-01, Vol.15 (2), p.346
Main Authors: Khaleeq, Sara, Sengupta, Nayanika, Kumar, Sahil, Patel, Unnatiben Rajeshbhai, Rajmani, Raju S, Reddy, Poorvi, Pandey, Suman, Singh, Randhir, Dutta, Somnath, Ringe, Rajesh P, Varadarajan, Raghavan
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Animals
Antibodies
Antigens
Bacterial proteins
Chromatography
Cloning
Coronaviruses
COVID-19
COVID-19 - prevention & control
Drug delivery systems
Drugs
encapsulin
Humans
Immunization
Immunogenicity
Influenza
Mice
Nanoparticles
Omicron
Peptides
Pharmaceutical research
Proteins
SARS-CoV-2 - genetics
Severe acute respiratory syndrome coronavirus 2
SpyCatcher
SpyTag
Squalene
SWE adjuvant
thermotolerant
Vaccines
Vehicles
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Summary:Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We have applied nanoparticle display combined with the SpyTag-SpyCatcher system to design encapsulin-mRBD, a nanoparticle vaccine displaying 180 copies of the monomeric SARS-CoV-2 spike receptor-binding domain (RBD). Here we show that encapsulin-mRBD is strongly antigenic and thermotolerant for long durations. After two immunizations, squalene-in-water emulsion (SWE)-adjuvanted encapsulin-mRBD in mice induces potent and comparable neutralizing antibody titers of 10 against wild-type (B.1), alpha, beta, and delta variants of concern. Sera also neutralizes the recent Omicron with appreciable neutralization titers, and significant neutralization is observed even after a single immunization.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15020346