Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis

Zhejiang and Sichuan are regarded as two genuine producing areas of Ophiopogonis radix in China. To study the difference in the quality of Ophiopogonis radix from these two places, the contents of three reported bioactive saponins, that is, ophiopogonins B, D, and D′, in tubers and fibrous roots of...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2016-01, Vol.2016 (2016), p.1-5
Main Authors: Liao, Dengqun, Sun, Peng, Wang, Yu-Xia, Li, Xian’en
Format: Article
Language:English
Subjects:
Analysis
Assaying
Biochemistry
Biocompatibility
Chemical properties
China
Chinese medicine
Detectors
High performance liquid chromatography
Mathematical analysis
Measurement
Medicine, Botanic
Medicine, Herbal
Metabolites
Methods
Provinces
Quality control
Quality standards
Roots
Saponins
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Summary:Zhejiang and Sichuan are regarded as two genuine producing areas of Ophiopogonis radix in China. To study the difference in the quality of Ophiopogonis radix from these two places, the contents of three reported bioactive saponins, that is, ophiopogonins B, D, and D′, in tubers and fibrous roots of Ophiopogon japonicus from Cixi city of Zhejiang and Santai county of Sichuan were quantified using HPLC-ELSD method and compared. Ophiopogonin B and ophiopogonin D′ content in tubers of HMD were higher than those in radix of CMD, whereas ophiopogonin D in HMD was about twice lower than that of CMD. Three ophiopogonins were all detected in fibrous roots of both HMD and CMD. Their averaged content in fibrous roots of CMD was about twice higher than that in tubers of CMD. Ophiopogonin D in fibrous roots of CMD was about five times higher than that of HMD. Our biochemical assay revealed that fibrous roots and tubers of CMD can be of an important saponin source, especially for ophiopogonin D.
ISSN:2090-8865
2090-8873
DOI:10.1155/2016/7214607