Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection

(CGMMV) is a member of the genus , which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on the whole plant, including leaves, stems, peduncles, and fruit. To better understand the molecular mechanisms of watermelon early responses to CGMMV infection, a comparativ...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-01, Vol.20 (3), p.610
Main Authors: Sun, Yuyan, Fan, Min, He, Yanjun
Format: Article
Language:English
Subjects:
Alanine
Ascorbic acid
Biosynthesis
Carotenoids
Chlorophyll
Chloroplasts
Circadian rhythms
Cucumber green mottle mosaic virus
Cutin
Dehydrogenases
Disease
Fatty acids
Flowers & plants
Galactose
Gene expression
Genomes
Infections
Isoleucine
Kinases
Leucine
Linolenic acid
Metabolism
Pathogens
Photosynthesis
Photosystem II
Proteins
qRT-PCR
Signal transduction
transcriptome
Tyrosine
Valine
Water melons
watermelon
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Summary:(CGMMV) is a member of the genus , which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on the whole plant, including leaves, stems, peduncles, and fruit. To better understand the molecular mechanisms of watermelon early responses to CGMMV infection, a comparative transcriptome analysis of 24 h CGMMV-infected and mock-inoculated watermelon leaves was performed. A total of 1641 differently expressed genes (DEGs) were identified, with 886 DEGs upregulated and 755 DEGs downregulated after CGMMV infection. A functional analysis indicated that the DEGs were involved in photosynthesis, plantā»pathogen interactions, secondary metabolism, and plant hormone signal transduction. In addition, a few transcription factor families, including WRKY, MYB, HLH, bZIP and NAC, were responsive to the CGMMV-induced stress. To confirm the high-throughput sequencing results, 15 DEGs were validated by qRT-PCR analysis. The results provide insights into the identification of candidate genes or pathways involved in the responses of watermelon leaves to CGMMV infection.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20030610