Speculation of Sphingolipids in Capsanthin by Ultra-Performance Liquid Chromatography Coupled with Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry

Sphingolipids are constituents of cellular membranes and play important roles in cells. As nutraceutical compounds in foods, sphingolipids have been proven to be critical for human health. Therefore, the sphingolipids content of capsanthin was established based on ultra-performance liquid chromatogr...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-01, Vol.28 (3), p.1010
Main Authors: Xu, Mei-Li, Qi, Lijun, Cai, Xingfu, Cao, Tong, Tang, Rumeng, Cao, Kaihang, Lian, Yunhe
Format: Article
Language:English
Subjects:
Analysis
Aqueous solutions
capsanthin
Cell membranes
ceramides
Chromatography
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
Communication
Composition
Electrospraying
Fatty Acids
Functional foods & nutraceuticals
glucosylceramides
Glucosylceramides - analysis
Health aspects
Humans
Ionization
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Methods
Quadrupoles
Scientific imaging
Spectrometry, Mass, Electrospray Ionization - methods
Sphingolipids
UPLC-Q-TOF HRMS/HRMS
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Summary:Sphingolipids are constituents of cellular membranes and play important roles in cells. As nutraceutical compounds in foods, sphingolipids have been proven to be critical for human health. Therefore, the sphingolipids content of capsanthin was established based on ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry. A total number of 40 sphingolipids were successfully identified, including 20 Glucosylceramides and 20 Ceramides. The predominant GlcCers contain 4-hydroxy-8-sphingenine t18:1 ( ) with different structures of α-OH fatty acids. For the Cers, the main long-chain bases are 4-hydroxy-8-sphingenine t18:1 ( ) and 4-hydroxysphingenine (t18:0) with different structures of α-OH or α, β-di (OH) fatty acids.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28031010