Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition

• Published in: BMC plant biology 2020-07, Vol.20 (1), p.319-23, Article 319
• Main Authors: Chakraborty, Joydeep, Sen, Senjuti, Ghosh, Prithwi, Jain, Akansha, Das, Sampa
• Format: Article
• Language: English
• Subjects: Apoptosis; Bioaccumulation; Cell death; Chickpeas; Cicer - genetics; Cicer - immunology; Cicer - microbiology; Cicer - physiology; Defense industry; DNA binding proteins; Flowers & plants; Fusarium - physiology; Fusarium oxysporum; Gene expression; Gene Expression Regulation, Plant - genetics; Genes; Genetic engineering; Genetically modified plants; Health aspects; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Immune response; Infection; Infections; Inoculation; Kinases; Legumes; Pathogens; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant genetics; Plant Immunity - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - immunology; Plant Roots - microbiology; Plant Roots - physiology; Plant Shoots - genetics; Plant Shoots - immunology; Plant Shoots - microbiology; Plant Shoots - physiology; Protein interaction; Protein Interaction Mapping; Protein-protein interaction; Protein-protein interactions; Proteins; R-protein signaling; Reactive oxygen species; Reactive oxygen species (ROS); Reactive Oxygen Species - metabolism; Roots; Salicylic acid; Salicylic Acid - administration & dosage; Signal transduction; Signal Transduction - genetics; Signal Transduction - immunology; Signaling; Systemic acquired resistance (SAR); Transcription activation; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptional regulation; Transgenic plants; Wilt
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-020-02527-9

Suppression and activation of plant defense genes is comprehensively regulated by WRKY family transcription factors. Chickpea, the non-model crop legume suffers from wilt caused by Fusarium oxysporum f. sp. ciceri Race1 (Foc1), defense response mechanisms of which are poorly understood. Here, we attempted to show interaction between WRKY70 and several downstream signaling components involved in susceptibility/resistance response in chickpea upon challenge with Foc1. In the present study, we found Cicer arietinum L. WRKY70 (CaWRKY70) negatively governs multiple defense responsive pathways, including Systemic Acquired Resistance (SAR) activation in chickpea upon Foc1 infection. CaWRKY70 is found to be significantly accumulated at shoot tissues of susceptible (JG62) chickpea under Foc1 stress and salicylic acid (SA) application. CaWRKY70 overexpression promotes susceptibility in resistant chickpea (WR315) plants to Foc1 infection. Transgenic plants upon Foc1 inoculation demonstrated suppression of not only endogenous SA concentrations but expression of genes involved in SA signaling. CaWRKY70 overexpressing chickpea roots exhibited higher ion-leakage and Foc1 biomass accumulation compared to control transgenic (VC) plants. CaWRKY70 overexpression suppresses H O production and resultant reactive oxygen species (ROS) induced cell death in Foc1 infected chickpea roots, stem and leaves. Being the nuclear targeted protein, CaWRKY70 suppresses CaMPK9-CaWRKY40 signaling in chickpea through its direct and indirect negative regulatory activities. Protein-protein interaction study revealed CaWRKY70 and CaRPP2-like CC-NB-ARC-LRR protein suppresses hyper-immune signaling in chickpea. Together, our study provides novel insights into mechanisms of suppression of the multiple defense signaling components in chickpea by CaWRKY70 under Foc1 stress. CaWRKY70 mediated defense suppression unveils networking between several immune signaling events negatively affecting downstream resistance mechanisms in chickpea under Foc1 stress.