Defective Atg16l1 in intestinal epithelial cells links to altered fecal microbiota and metabolic shifts during pregnancy in mice

• Published in: Gut microbes 2024-12, Vol.16 (1), p.2429267
• Main Authors: López-Agudelo, Víctor A., Falk-Paulsen, Maren, Bharti, Richa, Rehman, Ateequr, Sommer, Felix, Wacker, Eike Matthias, Ellinghaus, David, Luzius, Anne, Sievers, Laura Katharina, Liebeke, Manuel, Kaser, Arthur, Rosenstiel, Philip
• Format: Article
• Language: English
• Subjects: Animals; Atg16l1; Autophagy-Related Proteins - genetics; Autophagy-Related Proteins - metabolism; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Chemokine CXCL1 - genetics; Chemokine CXCL1 - metabolism; Crohn Disease - metabolism; Crohn Disease - microbiology; Epithelial Cells - metabolism; Epithelial Cells - microbiology; Feces - microbiology; Female; Gastrointestinal Microbiome; IBD; Intestinal Mucosa - metabolism; Intestinal Mucosa - microbiology; Metagenomics; Mice; Mice, Inbred C57BL; microbiota; Pregnancy; RNA, Ribosomal, 16S - genetics
• ISSN: 1949-0976; 1949-0984; 1949-0984
• DOI: 10.1080/19490976.2024.2429267

Throughout gestation, the female body undergoes a series of transformations, including profound alterations in intestinal microbial communities. Changes gradually increase toward the end of pregnancy and comprise reduced α-diversity of microbial communities and an increased propensity for energy harvest. Despite the importance of the intestinal microbiota for the pathophysiology of inflammatory bowel diseases, very little is known about the relationship between these microbiota shifts and pregnancy-associated complications of the disease. Here, we explored the longitudinal dynamics of gut microbiota composition and functional potential during pregnancy and after lactation in Atg16l1 ∆IEC mice carrying an intestinal epithelial deletion of the Crohn's disease risk gene Atg16l1. Using 16S rRNA amplicon and shotgun metagenomic sequencing, we demonstrated divergent temporal shifts in microbial composition between Atg16l1 wildtype and Atg16l1 ∆IEC pregnant mice in trimester 3, which was validated in an independent experiment. Observed differences included microbial genera implicated in IBD such as Lachnospiraceae, Roseburia, Ruminococcus, and Turicibacter. Changes partially recovered after lactation. Additionally, metagenomic and metabolomic analyses suggest an increased capacity for chitin degradation, resulting in higher levels of free N-acetyl-glucosamine products in feces, alongside reduced glucose and myo-inositol levels in serum around the time of delivery. On the host side, we found that the immunological response of Atg16l1 ∆IEC mice is characterized by higher colonic mRNA levels of TNFα and CXCL1 in trimester 3 and a lower weight of offspring at birth. Understanding pregnancy-dependent microbiome changes in the context of IBD may constitute the first step in the identification of fecal microbial biomarkers and microbiota-directed therapies that could help improve precision care for managing pregnancies in IBD patients.