Molecular typing and antimicrobial resistance of group B Streptococcus clinical isolates in Saudi Arabia

• Published in: Journal of global antimicrobial resistance. 2023-12, Vol.35, p.244-251
• Main Authors: Alzayer, Maha, Alkhulaifi, Manal M, Alyami, Ahmed, Aldosary, Mohammed, Alageel, Abdulaziz, Garaween, Ghada, Shibl, Atef, Al-Hamad, Arif M, Doumith, Michel
• Format: Article
• Language: English
• Subjects: Adult; Anti-Bacterial Agents - pharmacology; Antimicrobial resistance; Clindamycin - pharmacology; Drug Resistance, Bacterial; Erythromycin; Group B Streptococcus (GBS); Humans; Macrolides; MLVA typing; Molecular Typing; Pilus islands; Saudi Arabia - epidemiology; Serotypes; Serotyping; Streptococcal Infections - epidemiology; Streptococcus agalactiae
• ISSN: 2213-7165; 2213-7173
• DOI: 10.1016/j.jgar.2023.10.007

Group B Streptococcus (GBS) has emerged as an important cause of severe infections in adults. However, limited data are available regarding the epidemiology of GBS in Saudi Arabia. Isolates were collected over a period of eight months from colonized (n = 104) and infected adults (n = 95). Serotypes and virulence determinants were detected by polymerase chain reactions (PCRs). Genetic relatedness was assessed using Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). Antimicrobial susceptibilities were determined by disk diffusion. Serotypes III and V (25% each) were the most prevalent, followed by serotypes II (16.18%), Ia (13.24%), VI (9.31%), and Ib (8.82%), while five isolates remained non-typeable (2.45%). Hypervirulent serotype III/CC17 clone (n = 21) accounted for 41.18% of the serotype III isolates. Most isolates (53.92%) harboured pilus island (PI) 1 and 2a types, while PI-2b was predominantly detected in the hypervirulent clone. Isolates were variably resistant to tetracycline (76.47%), erythromycin (36.76%), clindamycin (25.49%), and levofloxacin (6.37%), but remained susceptible to penicillin. Macrolide resistant isolates exhibited constitutive (55.42%) and inducible macrolide-lincosamide-streptogramin B resistance phenotypes (33.74%), while a few had L (9.64%) or M (1.2%) phenotypes. MLVA patterns of dominant serotypes III and V revealed 40 different types divided into 12 clusters and 28 singletons. Interestingly, macrolide resistance was significantly associated with two major MLVA types. GBS isolates belonged predominantly to serotypes III and V, but there were no clear associations between serotypes and patient groups. The studied isolates exhibited high levels of resistance to erythromycin and clindamycin that need further surveillance.