Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae

• Published in: Frontiers in nutrition (Lausanne) 2022-06, Vol.9, p.911310-911310
• Main Authors: Tang, Zizhong, Lin, Wenjie, Chen, Yusheng, Feng, Shiling, Qin, Yihan, Xiao, Yirong, Chen, Hong, Liu, Yuntao, Chen, Hui, Bu, Tongliang, Li, Qinfeng, Cai, Yi, Yao, Huipeng, Ding, Chunbang
• Format: Article
• Language: English
• Subjects: Cordyceps cicadae (C. cicadae); extraction; Nutrition; physicochemical properties; polysaccharide; purification
• ISSN: 2296-861X; 2296-861X
• DOI: 10.3389/fnut.2022.911310

The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from C. cicadae was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro . In addition, JCH-a1 can prolong the lifespan of C. elegans . The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.