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Toxicity study of rats treated by plasma-activated solution
Cold atmospheric plasma (CAP) is an effective treatment for various skin diseases. Plasma-activated solution (PAS) is an indirect method of CAP treatment that produces biological effects similar to those of direct treatment with plasma devices. The anticancer and bacteriostatic effects of PAS have b...
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Published in: | Heliyon 2023-12, Vol.9 (12), p.e23116-e23116, Article e23116 |
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description | Cold atmospheric plasma (CAP) is an effective treatment for various skin diseases. Plasma-activated solution (PAS) is an indirect method of CAP treatment that produces biological effects similar to those of direct treatment with plasma devices. The anticancer and bacteriostatic effects of PAS have been demonstrated in vitro experiments; however, on the basis of the lack of toxicological studies on PAS, its effects on living mammals when administered by subcutaneous injection is poorly known.
The purpose of this study was to evaluate the effects of PAS on local skin tissue cells, blood system, heart, liver, lungs, kidneys and other vital organs of the rat when injected subcutaneously.
PAS was prepared by CAP irradiation of phosphate-buffered saline (PBS). PBS and different PBS groups (CAP irradiation for 1, 3, or 5 min) were injected subcutaneously once every 48 h. The rats were euthanized immediately after 10 cycles of therapy.
No adverse effects were observed during the entire period of the experiment. Histopathological examination of organs and tissues revealed no structural changes. Moreover, no obvious structural changes were observed in skin tissue. DNA damage and cancerous proliferative changes were not detected in skin tissue treated with PAS. Subsequently, RNA sequencing and western blotting were performed. The results showed that PAS increased the expression of growth factors like transforming growth factor beta (TGF-β) and vascular endothelial growth factor A (VEGFA). These results might be directly linked to the role of PAS in stimulating TGF-β receptor signaling pathway and angiogenesis.
The results showed that multiple subcutaneous injections of PAS did not show significant toxic side effects on local skin tissues and some vital organs in rats, providing a scientific basis to support the future treatment of skin diseases with PAS.
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doi_str_mv | 10.1016/j.heliyon.2023.e23116 |
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The purpose of this study was to evaluate the effects of PAS on local skin tissue cells, blood system, heart, liver, lungs, kidneys and other vital organs of the rat when injected subcutaneously.
PAS was prepared by CAP irradiation of phosphate-buffered saline (PBS). PBS and different PBS groups (CAP irradiation for 1, 3, or 5 min) were injected subcutaneously once every 48 h. The rats were euthanized immediately after 10 cycles of therapy.
No adverse effects were observed during the entire period of the experiment. Histopathological examination of organs and tissues revealed no structural changes. Moreover, no obvious structural changes were observed in skin tissue. DNA damage and cancerous proliferative changes were not detected in skin tissue treated with PAS. Subsequently, RNA sequencing and western blotting were performed. The results showed that PAS increased the expression of growth factors like transforming growth factor beta (TGF-β) and vascular endothelial growth factor A (VEGFA). These results might be directly linked to the role of PAS in stimulating TGF-β receptor signaling pathway and angiogenesis.
The results showed that multiple subcutaneous injections of PAS did not show significant toxic side effects on local skin tissues and some vital organs in rats, providing a scientific basis to support the future treatment of skin diseases with PAS.
[Display omitted]</description><identifier>ISSN: 2405-8440</identifier><identifier>EISSN: 2405-8440</identifier><identifier>DOI: 10.1016/j.heliyon.2023.e23116</identifier><identifier>PMID: 38144323</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Angiogenesis ; Cold atmospheric plasma ; Immunohistochemistry ; Plasma-activated solution ; Safety study</subject><ispartof>Heliyon, 2023-12, Vol.9 (12), p.e23116-e23116, Article e23116</ispartof><rights>2023 The Authors</rights><rights>2023 The Authors. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-6a4bf422ac7c6c0df1db1d5fbee806fe2da8cbdd81ea0406f4462b64f0c1e2a83</citedby><cites>FETCH-LOGICAL-c478t-6a4bf422ac7c6c0df1db1d5fbee806fe2da8cbdd81ea0406f4462b64f0c1e2a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S2405844023103240$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38144323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Fanfan</creatorcontrib><creatorcontrib>He, Houyu</creatorcontrib><creatorcontrib>Wang, Liyun</creatorcontrib><creatorcontrib>Yang, Xingyu</creatorcontrib><creatorcontrib>Gao, Jing</creatorcontrib><creatorcontrib>Wang, Jingwen</creatorcontrib><creatorcontrib>Zhang, Shengquan</creatorcontrib><creatorcontrib>Huang, Shenghai</creatorcontrib><creatorcontrib>Yang, Chunjun</creatorcontrib><title>Toxicity study of rats treated by plasma-activated solution</title><title>Heliyon</title><addtitle>Heliyon</addtitle><description>Cold atmospheric plasma (CAP) is an effective treatment for various skin diseases. Plasma-activated solution (PAS) is an indirect method of CAP treatment that produces biological effects similar to those of direct treatment with plasma devices. The anticancer and bacteriostatic effects of PAS have been demonstrated in vitro experiments; however, on the basis of the lack of toxicological studies on PAS, its effects on living mammals when administered by subcutaneous injection is poorly known.
The purpose of this study was to evaluate the effects of PAS on local skin tissue cells, blood system, heart, liver, lungs, kidneys and other vital organs of the rat when injected subcutaneously.
PAS was prepared by CAP irradiation of phosphate-buffered saline (PBS). PBS and different PBS groups (CAP irradiation for 1, 3, or 5 min) were injected subcutaneously once every 48 h. The rats were euthanized immediately after 10 cycles of therapy.
No adverse effects were observed during the entire period of the experiment. Histopathological examination of organs and tissues revealed no structural changes. Moreover, no obvious structural changes were observed in skin tissue. DNA damage and cancerous proliferative changes were not detected in skin tissue treated with PAS. Subsequently, RNA sequencing and western blotting were performed. The results showed that PAS increased the expression of growth factors like transforming growth factor beta (TGF-β) and vascular endothelial growth factor A (VEGFA). These results might be directly linked to the role of PAS in stimulating TGF-β receptor signaling pathway and angiogenesis.
The results showed that multiple subcutaneous injections of PAS did not show significant toxic side effects on local skin tissues and some vital organs in rats, providing a scientific basis to support the future treatment of skin diseases with PAS.
[Display omitted]</description><subject>Angiogenesis</subject><subject>Cold atmospheric plasma</subject><subject>Immunohistochemistry</subject><subject>Plasma-activated solution</subject><subject>Safety study</subject><issn>2405-8440</issn><issn>2405-8440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqFkUFv3CAQhVHUKonS_IRWPvbiDWCMsXqoqqhNIkXqJT2jAYaWlXfZAo7qf18Sb6LeegI9vfkezCPkPaMbRpm82m5-4RSWuN9wyrsN8o4xeULOuaB9q4Sgb_65n5HLnLeUUtYrOQ7dKTnrFBOi4905-fQQ_wQbytLkMrulib5JUHJTEkJB15ilOUyQd9CCLeHxWctxmkuI-3fkrYcp4-XxvCA_vn19uL5t77_f3F1_uW-tGFRpJQjjBedgBystdZ45w1zvDaKi0iN3oKxxTjEEKqoihORGCk8tQw6quyB3K9dF2OpDCjtIi44Q9LMQ008NqQQ7ofYUqHSDN9Y5Uckj8AEc5WOlGyfGyvq4sg4p_p4xF70L2eI0wR7jnDUfaT-orj68WvvValPMOaF_jWZUP9Wgt_pYg36qQa811LkPx4jZ7NC9Tr0svRo-rwasS3sMmHS2AfcWXUhoS_1V-E_EX8wjnP4</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Chen, Fanfan</creator><creator>He, Houyu</creator><creator>Wang, Liyun</creator><creator>Yang, Xingyu</creator><creator>Gao, Jing</creator><creator>Wang, Jingwen</creator><creator>Zhang, Shengquan</creator><creator>Huang, Shenghai</creator><creator>Yang, Chunjun</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>202312</creationdate><title>Toxicity study of rats treated by plasma-activated solution</title><author>Chen, Fanfan ; He, Houyu ; Wang, Liyun ; Yang, Xingyu ; Gao, Jing ; Wang, Jingwen ; Zhang, Shengquan ; Huang, Shenghai ; Yang, Chunjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-6a4bf422ac7c6c0df1db1d5fbee806fe2da8cbdd81ea0406f4462b64f0c1e2a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Angiogenesis</topic><topic>Cold atmospheric plasma</topic><topic>Immunohistochemistry</topic><topic>Plasma-activated solution</topic><topic>Safety study</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Fanfan</creatorcontrib><creatorcontrib>He, Houyu</creatorcontrib><creatorcontrib>Wang, Liyun</creatorcontrib><creatorcontrib>Yang, Xingyu</creatorcontrib><creatorcontrib>Gao, Jing</creatorcontrib><creatorcontrib>Wang, Jingwen</creatorcontrib><creatorcontrib>Zhang, Shengquan</creatorcontrib><creatorcontrib>Huang, Shenghai</creatorcontrib><creatorcontrib>Yang, Chunjun</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Heliyon</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Fanfan</au><au>He, Houyu</au><au>Wang, Liyun</au><au>Yang, Xingyu</au><au>Gao, Jing</au><au>Wang, Jingwen</au><au>Zhang, Shengquan</au><au>Huang, Shenghai</au><au>Yang, Chunjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxicity study of rats treated by plasma-activated solution</atitle><jtitle>Heliyon</jtitle><addtitle>Heliyon</addtitle><date>2023-12</date><risdate>2023</risdate><volume>9</volume><issue>12</issue><spage>e23116</spage><epage>e23116</epage><pages>e23116-e23116</pages><artnum>e23116</artnum><issn>2405-8440</issn><eissn>2405-8440</eissn><abstract>Cold atmospheric plasma (CAP) is an effective treatment for various skin diseases. Plasma-activated solution (PAS) is an indirect method of CAP treatment that produces biological effects similar to those of direct treatment with plasma devices. The anticancer and bacteriostatic effects of PAS have been demonstrated in vitro experiments; however, on the basis of the lack of toxicological studies on PAS, its effects on living mammals when administered by subcutaneous injection is poorly known.
The purpose of this study was to evaluate the effects of PAS on local skin tissue cells, blood system, heart, liver, lungs, kidneys and other vital organs of the rat when injected subcutaneously.
PAS was prepared by CAP irradiation of phosphate-buffered saline (PBS). PBS and different PBS groups (CAP irradiation for 1, 3, or 5 min) were injected subcutaneously once every 48 h. The rats were euthanized immediately after 10 cycles of therapy.
No adverse effects were observed during the entire period of the experiment. Histopathological examination of organs and tissues revealed no structural changes. Moreover, no obvious structural changes were observed in skin tissue. DNA damage and cancerous proliferative changes were not detected in skin tissue treated with PAS. Subsequently, RNA sequencing and western blotting were performed. The results showed that PAS increased the expression of growth factors like transforming growth factor beta (TGF-β) and vascular endothelial growth factor A (VEGFA). These results might be directly linked to the role of PAS in stimulating TGF-β receptor signaling pathway and angiogenesis.
The results showed that multiple subcutaneous injections of PAS did not show significant toxic side effects on local skin tissues and some vital organs in rats, providing a scientific basis to support the future treatment of skin diseases with PAS.
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subjects | Angiogenesis Cold atmospheric plasma Immunohistochemistry Plasma-activated solution Safety study |
title | Toxicity study of rats treated by plasma-activated solution |
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