Genome Analysis and Description of Three Novel Diazotrophs Geomonas Species Isolated From Paddy Soils

Five strictly anaerobic strains, designated RG2 , RG3, RG10 , RF4 , and RG29, were isolated from paddy soils in China. Strains RG2 , RF4 , RG10 , RG3, and RG29 grew at temperatures ranging 5-42°C and pH ranging 5.5-8.5. Strains RG2 , RF4 , RG3, and RG29 could tolerate NaCl up to 0-0.7% (w/v) while s...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2022-02, Vol.12, p.801462-801462
Main Authors: Liu, Guo-Hong, Yang, Shang, Tang, Rong, Xie, Cheng-Jie, Zhou, Shun-Gui
Format: Article
Language:English
Subjects:
Geomonas nitrogeniifigens
Geomonas oryzisoli
Geomonas subterranea
iron reduction
Microbiology
nitrogen fixation activity
paddy soil
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Five strictly anaerobic strains, designated RG2 , RG3, RG10 , RF4 , and RG29, were isolated from paddy soils in China. Strains RG2 , RF4 , RG10 , RG3, and RG29 grew at temperatures ranging 5-42°C and pH ranging 5.5-8.5. Strains RG2 , RF4 , RG3, and RG29 could tolerate NaCl up to 0-0.7% (w/v) while strain RG10 could tolerate NaCl up to 0-0.8% (w/v). The isolated strains showed the highest 16S rRNA gene sequence similarities to the type strains of Red111 and Red736 . In phylogenetic (based on 16S rRNA gene sequence) and phylogenomic trees, strains clustered with the members of the genus Menaquinone-8 was the predominant quinone present in all strains. The major fatty acid profiles of all strains were C ω6 , C , iso-C , and Summed Feature 3. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the isolated strains and the closely related species were lower than the cutoff value (ANI 95-96% and dDDH 70%) for prokaryotic species delineation. Based on physiological, biochemical, and chemotaxonomic properties, strains RG2 , RG10 , and RF4 could easily be differentiated with the members of the genus . Additionally, all the isolated strains possessed clusters and catalytic compartments of nitrogenase. Based on the above results, the isolated five strains represent three novel species of the genus , for which the names sp. nov., sp. nov., and sp. nov. are proposed. The type strains are RG10 (= GDMCC1.2537 = KCTC 26318 ), RG2 (= GDMCC1.2536 = KCTC 25317 ), and RF4 (= GDMCC 1.2547 = KCTC 25316 ).
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.801462