Cotyledonary somatic embryo is one kind of intermediate material similar to callus in the process of in vitro tissue culture from Rosa hybrida 'John F. Kennedy'

• Published in: BMC genomics 2024-04, Vol.25 (1), p.362-362, Article 362
• Main Authors: Du, Li, Kang, Xiaoling, Guo, Haoran, Zhu, Zhongfeng, Wu, Rui, Yuan, Meijing, Ding, Chuanyu
• Format: Article
• Language: English
• Subjects: Analysis; Biological activity; Biosynthesis; Callus; Cell culture; Cell differentiation; Cell walls; Cellular signal transduction; Chlorophyll; Cytochrome; Developmental biology; Differentiation (biology); Diseases and pests; Down-regulation; Embryo; Embryonic Development; Embryonic growth stage; Embryos; Ethylene; Ethylenes; Explants; Fatty acids; Flowers & plants; Gangliosides; Genes; Genetic aspects; Genetic transcription; Genetic transformation; Genomes; Genomics; Germination; Gibberellins; Growth; Intermediate material in vitro tissue culture; Kennedy, John F; Kennedy, John Fitzgerald (1917-1963); Metabolism; Methods; Molecular modelling; Morphology; Organogenesis; Ornamental plants; Phosphatases; Phosphorylation; Photosynthesis; Plant breeding; Plant Growth Regulators; Plant hormones; Porphyrins; Protein phosphatase; Quinones; Receptors; Redox reactions; Regeneration; Rosa - genetics; Rosa hybrida; Rosa hybrida ‘John F. Kennedy’; Roses; Seed propagation and vegetative propagation; Sequences; Signal transduction; Single-piece cotyledonary somatic embryo culture; Somatic embryogenesis; Somatic embryogenesis and organogenesis; Tissue culture; Transcription Factors; Transcriptomes
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10256-8

Rose is recognized as an important ornamental plant worldwide, and it is also one of the most widely used flowers in gardens. At present, the improvement of rose traits is still difficult and uncertain, and molecular breeding can provide new ideas for the improvement of modern rose varieties. Somatic embryos are quite good receptors for genetic transformation. However, little is known about the molecular mechanisms underlying during the regeneration process of rose somatic embryos. To elucidate the molecular regulation mechanism of somatic embryo plantlet regeneration, the relationship between the differences in traits of the two different regenerated materials and the significantly differentially expressed genes (DEGs) related to phytohormone pathways in the process of regeneration were be investigated. These representative two regenerated samples from single-piece cotyledonary somatic embryo (SPC) culture of Rosa hybrida 'John F. Kennedy', were harvested for transcriptome analysis, with the SPC explants at the initial culture (Day 0) as the control. The differentially expressed genes (DEGs) in the materials from two different types for regeneration approach (SBF type: the regeneration approach type of single bud formed from SPC explants; MBF type: the regeneration approach type of multiple buds formed from SPC explants) were be screened by means of the transcriptome sequencing technology. In this study, a total of about 396.24 million clean reads were obtained, of which 78.95-82.92% were localized to the reference genome, compared with the initial material (CK sample), there were 5594 specific genes in the material of SBF type and 6142 specific genes in the MBF type. The DEGs from the SBF type material were mainly concentrated in the biological processes of GO terms such as phytohormones, substance transport, cell differentiation, and redox reaction. The KEGG enrichment analysis revealed these DEGs were more active in ubiquinone and other terpenoid-quinone biosynthesis, fatty acid elongation, steroid biosynthesis, and glycosphingolipid biosynthesis-globo and isoglobo series. In contrast, the DEGs induced by the MBF type material were mainly associated with the biological processes such as phytohormones, phosphorylation, photosynthesis and signal transduction. According to KEGG analysis, these DEGs of MBF type were significantly enriched in the porphyrin and chlorophyll metabolism, brassinosteroid biosynthesis, carotenoid biosynthesis, and peroxisome. Fur