New Insights into Endogenous Retrovirus-K Transcripts in Amyotrophic Lateral Sclerosis

Retroviral reverse transcriptase activity and the increased expression of human endogenous retroviruses (HERVs) are associated with amyotrophic lateral sclerosis (ALS). We were interested in confirming overexpression in the ALS brain, its use as an accessory diagnostic marker for ALS, and its potent...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2024-02, Vol.25 (3), p.1549
Main Authors: Moreno-Martinez, Laura, Macías-Redondo, Sofía, Strunk, Mark, Guillén-Antonini, María Isabel, Lunetta, Christian, Tarlarini, Claudia, Penco, Silvana, Calvo, Ana Cristina, Osta, Rosario, Schoorlemmer, Jon
Format: Article
Language:English
Subjects:
Amyotrophic lateral sclerosis
Amyotrophic Lateral Sclerosis - metabolism
Analysis
Brain - metabolism
Brain Stem - metabolism
copy-specific expression
Cytokines
Development and progression
Diagnosis
Disease
DNA polymerases
endogenous retroviruses
Endogenous Retroviruses - genetics
Genomes
Humans
Leukocytes, Mononuclear - metabolism
Mutation
Neurotoxicity
NLRP3
Pathogenesis
quantitative PCR
RNA polymerase
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Retroviral reverse transcriptase activity and the increased expression of human endogenous retroviruses (HERVs) are associated with amyotrophic lateral sclerosis (ALS). We were interested in confirming overexpression in the ALS brain, its use as an accessory diagnostic marker for ALS, and its potential interplay with neuroinflammation. Using qPCR to analyze expression in peripheral blood mononuclear cells (PBMCs) and in postmortem brain samples from ALS patients, no significant differences were observed between patients and control subjects. By contrast, we report alterations in the expression patterns of specific copies, especially in the brainstem. Out of 27 copies sampled, the relative expression of 17 was >1.2-fold changed in samples from ALS patients. In particular, the relative expression of two copies (Chr3-3 and Chr3-5) was significantly different in brainstem samples from ALS patients compared with controls. Further qPCR analysis of inflammation markers in brain samples revealed a significant increase in levels, while , , and showed slight decreases. We cannot confirm global overexpression in ALS, but we can report the ALS-specific overexpression of selected copies in the ALS brain. Our data are compatible with the requirement for better patient stratification and support the potential importance of particular copies in ALS.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25031549