Molecular characterization of Toxoplasma gondii isolates from free-range chickens reveals new genotypes in Goiânia, Goiás, Brazil

The aim of this study was to evaluate the genotypic characteristics of Toxoplasma gondii isolated from free-range chickens in the metropolitan area of Goiânia, Goiás, in Brazil's central-west region. The seroprevalence rate was found to be 96%, according to an indirect hemagglutination assay. B...

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Published in:Revista brasileira de parasitologia veterinaria 2021-01, Vol.30 (2), p.e000321-e000321
Main Authors: Rezende, Hanstter Hallison Alves, Igreja, Jaqueline Ataíde Silva Lima da, Gomes-Júnior, Antônio Roberto, Melo, Jade de Oliveira, Garcia, João Luís, Martins, Felippe Danyel Cardoso, Storchilo, Heloísa Ribeiro, Gomes, Taynara Cristina, Vinaud, Marina Clare, Castro, Ana Maria de
Format: Article
Language:English
Subjects:
Animals
bioassay
Brazil
Chickens
free-range chickens
genetic diversity
Genetic Variation
Genotype
Mice
molecular characterization
PARASITOLOGY
PCR-RFLP
Seroepidemiologic Studies
Toxoplasma - genetics
Toxoplasma gondii
Toxoplasmosis, Animal - diagnosis
Toxoplasmosis, Animal - epidemiology
VETERINARY SCIENCES
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Summary:The aim of this study was to evaluate the genotypic characteristics of Toxoplasma gondii isolated from free-range chickens in the metropolitan area of Goiânia, Goiás, in Brazil's central-west region. The seroprevalence rate was found to be 96%, according to an indirect hemagglutination assay. Brain and heart samples were processed by peptic digestion for a mice bioassay. The tissues were homogenized and the resulting samples were subjected to polymerase chain reaction (PCR), which revealed that 64% of them contained the parasite's DNA. The mice bioassay revealed 15 isolates, 8 of them tachyzoites isolates from the peritoneal lavage and 7 from brain cysts. T. gondii genotypes were determined through PCR-RFLP, using the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, alt. SAG2, Apico and CS3. Three genotypes were identified, inclued ToxoDB #65, and the other two are not yet described in the literature. Hence, we conclude that the isolates obtained from the metropolitan area of Goiânia showed relatively low genetic diversity.
ISSN:0103-846X
1984-2961
1984-2961
DOI:10.1590/S1984-29612021029