Diet-Induced Obesity and Ghrelin Effects on Pituitary Gonadotrophs: Immunohistomorphometric Study in Male Rats

The close relationship between energy metabolism, nutritional state, and reproductive physiology suggests that nutritional and metabolic disorders can disrupt normal reproductive function and fertility. Considering the importance of leptin and ghrelin effects in regulation of the hypothalamic-pituit...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2016-01, Vol.17 (4), p.711-719
Main Authors: Ristic, Natasa, Stevanovic, Darko, Nesic, Dejan, Ajdzanovic, Vladimir, Rakocevic, Rastko, Jaric, Ivana, Milosevic, Verica
Format: Article
Language:English
Subjects:
Ghrelin
Gonadotrophs
Male
Metabolic disorders
Obesity
Original
Pituitary gland
Rats
Rodents
Studies
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Summary:The close relationship between energy metabolism, nutritional state, and reproductive physiology suggests that nutritional and metabolic disorders can disrupt normal reproductive function and fertility. Considering the importance of leptin and ghrelin effects in regulation of the hypothalamic-pituitary-gonadal axis, the objective of this study was to investigate the influence of obesity and centrally applied ghrelin on immunohistochemical appearance and quantitative morphology of the pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH) producing cells in adult male rats. In this experimental study, animals were given two differ- ent diets: normal-fat (NF) and high-fat (HF), for 4 weeks, corresponding to normal and positive energy balance (n=2×14), respectively. Each group was subsequently divided into two subgroups (n=7) receiving intracerebroventricular (ICV) injections of either ghrelin [G, 1 µg/5 µL phosphate buffered saline (PBS)] or vehicle (5 µL PBS, control group) every 24 hours for five consecutive days. Morphometric analyses showed that in HF control group, the percentage of FSH cells per unit volume of total pituitary gland tissue (in μm(3)), i.e. volume density (Vvc), was increased (P
ISSN:2228-5806
2228-5814