Understanding the flow behavior around marine biofilms

• Published in: Biofilm 2024-06, Vol.7, p.100204, Article 100204
• Main Authors: Romeu, Maria J., Miranda, João M., de Jong, Ed. D., Morais, João, Vasconcelos, Vítor, Sjollema, Jelmer, Mergulhão, Filipe J.
• Format: Article
• Language: English
• Subjects: Biofilm structural parameters; Biofilms; CFD modelling; Hydrodynamic conditions; Marine biofouling; OCT; Shear forces
• ISSN: 2590-2075; 2590-2075
• DOI: 10.1016/j.bioflm.2024.100204

In vitro platforms capable of mimicking the hydrodynamic conditions prevailing in natural aquatic environments have been previously validated and used to predict the fouling behavior on different surfaces. Computational Fluid Dynamics (CFD) has been used to predict the shear forces occurring in these platforms. In general, these predictions are made for the initial stages of biofilm formation, where the amount of biofilm does not affect the flow behavior, enabling the estimation of the shear forces that initial adhering organisms have to withstand. In this work, we go a step further in understanding the flow behavior when a mature biofilm is present in such platforms to better understand the shear rate distribution affecting marine biofilms. Using 3D images obtained by Optical Coherence Tomography, a mesh was produced and used in CFD simulations. Biofilms of two different marine cyanobacteria were developed in agitated microtiter plates incubated at two different shaking frequencies for 7 weeks. The biofilm-flow interactions were characterized in terms of the velocity field and shear rate distribution. Results show that global hydrodynamics imposed by the different shaking frequencies affect biofilm architecture and also that this architecture affects local hydrodynamics, causing a large heterogeneity in the shear rate field. Biofilm cells located in the streamers of the biofilm are subjected to much higher shear values than those located on the bottom of the streamers and this dispersion in shear rate values increases at lower bulk fluid velocities. This heterogeneity in the shear force field may be a contributing factor for the heterogeneous behavior in metabolic activity, growth status, gene expression pattern, and antibiotic resistance often associated with nutrient availability within the biofilm. •Biofilm development was higher at the lowest shaking frequency.•Rougher biofilms induce larger perturbations in flow velocities around biofilms.•Shear rate field was more heterogeneous for biofilms formed at the lowest rotation.•Shear rate heterogeneity may contribute to heterogeneous cell behavior in biofilms.