Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread

Diarrhea is the third leading infectious cause of death worldwide and is estimated to be responsible for approximately 2 million deaths a year. While many infectious causes of diarrhea have been established, approximately 40% of all diarrhea cases are of unknown etiology. In an effort to identify no...

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Published in:Virology journal 2009-06, Vol.6 (86), p.86-86, Article 86
Main Authors: Holtz, Lori R, Finkbeiner, Stacy R, Zhao, Guoyan, Kirkwood, Carl D, Girones, Rosina, Pipas, James M, Wang, David
Format: Article
Language:English
Subjects:
Australia
Child, Preschool
Diarrea
Diarrhea
DNA sequencing
Feces - virology
Gastroenteritis - virology
Genetic aspects
Genome, Viral
Health aspects
Humans
Malalties víriques
Methods
Molecular Sequence Data
Nucleotide sequencing
Phylogeny
Picornaviridae - classification
Picornaviridae - genetics
Picornaviridae - isolation & purification
Picornaviridae Infections - virology
Picornaviruses
Risk factors
RNA, Viral - genetics
Sequence Analysis, DNA
Sewage - virology
Spain
United States
Virus diseases
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Summary:Diarrhea is the third leading infectious cause of death worldwide and is estimated to be responsible for approximately 2 million deaths a year. While many infectious causes of diarrhea have been established, approximately 40% of all diarrhea cases are of unknown etiology. In an effort to identify novel viruses that may be causal agents of diarrhea, we used high throughput mass sequencing to analyze stool samples collected from patients with acute diarrhea. Sequences with limited similarity to known picornaviruses were detected in a stool sample collected in Australia from a child with acute diarrhea. Using a combination of mass sequencing, RT-PCR, 5' RACE and 3' RACE, a 6383 bp fragment of the viral genome was sequenced. Phylogenetic analysis demonstrated that this virus was highly divergent from, but most closely related to, members of the genus Kobuvirus. We have tentatively named this novel virus klassevirus 1. We also detected klassevirus 1 by RT-PCR in a diarrhea specimen collected from a patient in St. Louis, United States as well as in untreated sewage collected in Barcelona, Spain. Klassevirus 1 is a previously undescribed picornavirus that is globally widespread and present on at least three continents. Further investigations to determine whether klassevirus 1 is a human pathogen are needed.
ISSN:1743-422X
1743-422X
DOI:10.1186/1743-422x-6-86