Systemic β-Adrenergic Receptor Activation Augments the ex vivo Expansion and Anti-Tumor Activity of Vγ9Vδ2 T-Cells

• Published in: Frontiers in immunology 2020-01, Vol.10, p.3082-3082
• Main Authors: Baker, Forrest L, Bigley, Austin B, Agha, Nadia H, Pedlar, Charles R, O'Connor, Daniel P, Bond, Richard A, Bollard, Catherine M, Katsanis, Emmanuel, Simpson, Richard J
• Format: Article
• Language: English
• Subjects: adoptive transfer immunotherapy; Adrenergic beta-Agonists - administration & dosage; Adrenergic beta-Antagonists - administration & dosage; Adult; beta-blockers; Bisoprolol - administration & dosage; Cell Line; Cell Proliferation; Cytotoxicity, Immunologic; exercise; Exercise - physiology; exercise immunology; Female; gamma-delta t-cells; Graft vs Tumor Effect - immunology; Hematopoietic Stem Cell Transplantation; Humans; Immunology; Immunotherapy, Adoptive - methods; Lymphocyte Activation; Male; Nadolol - administration & dosage; Neoplasms - immunology; Neoplasms - therapy; Receptors, Adrenergic - metabolism; Receptors, Antigen, T-Cell, gamma-delta - metabolism; T-Lymphocytes - immunology; Transplantation, Homologous; Young Adult
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2019.03082

TCR-gamma delta (γδ) T-cells are considered important players in the graft-vs.-tumor effect following allogeneic hematopoietic cell transplantation (alloHCT) and have emerged as candidates for adoptive transfer immunotherapy in the treatment of both solid and hematological tumors. Systemic β-adrenergic receptor (β-AR) activation has been shown to mobilize TCR-γδ T-cells to the blood, potentially serving as an adjuvant for alloHCT and TCR-γδ T-cell therapy. We investigated if systemic β-AR activation, using acute dynamic exercise as an experimental model, can increase the mobilization, expansion, and anti-tumor activity of TCR-γδ T-cells isolated from the blood of healthy humans. We also sought to investigate the β-AR subtypes involved, by administering a preferential β -AR antagonist (bisoprolol) and a non-preferential β + β -AR antagonist (nadolol) prior to exercise as part of a randomized placebo controlled cross-over experiment. We found that exercise mobilized TCR-γδ cells to blood and augmented their expansion by ~182% compared to resting blood when stimulated with IL-2 and ZOL for 14-days. Exercise also increased the proportion of CD56+, NKG2D+/CD62L-, CD158a/b/e+ and NKG2A- cells among the expanded TCR-γδ cells, and increased their cytotoxic activity against several tumor target cells (K562, U266, 221.AEH) by 40-60%. Blocking NKG2D on TCR-γδ cells eliminated the augmented cytotoxic effects of exercise against U266 target cells. Furthermore, administering a β + β -AR (nadolol), but not a β -AR (bisoprolol) antagonist prior to exercise abrogated the exercise-induced enhancement in TCR-γδ T-cell mobilization and expansion. Furthermore, nadolol completely abrogated while bisoprolol partially inhibited the exercise-induced increase in the cytotoxic activity of the expanded TCR-γδ T-cells. We conclude that acute systemic β-AR activation in healthy donors markedly augments the mobilization, expansion, and anti-tumor activity of TCR-γδ T-cells and that some of these effects are due to β -AR signaling and phenotypic shifts that promote a dominant activating signal via NKG2D. These findings highlight β-ARs as potential targets to favorably alter the composition of allogeneic peripheral blood stem cell grafts and improve the potency of TCR-γδ T-cell immune cell therapeutics.