Analysis of In Vivo Existence Forms of Nardosinone in Mice by UHPLC-Q-TOF-MS Technique

Nardosinone, a sesquiterpene peroxide, is one of the main active constituents of the ethnomedicine Nardostachyos Radix et Rhizoma, and it has many bioactivities, such as antiarrhythmia and cardioprotection. To elucidate its in vivo existence forms, its metabolism is first studied using mice. All uri...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-10, Vol.27 (21), p.7267
Main Authors: Zhang, Jing, Lv, Yang, Bai, Yu-Sha, Li, Meng-Yuan, Wang, Shun-Qi, Wang, Li-Li, Liu, Guang-Xue, Xu, Feng, Shang, Ming-Ying, Cai, Shao-Qing
Format: Article
Language:English
Subjects:
Acids
Analysis
Anti-arrhythmia drugs
Carbon
Carboxylation
Dehydration
Demethylation
Dosage
existence form
Feces
Hydroxylation
in vivo metabolism
In vivo methods and tests
Isomers
Malaria
Metabolism
Metabolites
Methods
nardosinone
Oral administration
Parkinson's disease
Physiological aspects
sesquiterpene peroxide
Sulfation
UHPLC-Q-TOF-MS
Urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nardosinone, a sesquiterpene peroxide, is one of the main active constituents of the ethnomedicine Nardostachyos Radix et Rhizoma, and it has many bioactivities, such as antiarrhythmia and cardioprotection. To elucidate its in vivo existence forms, its metabolism is first studied using mice. All urine and feces are collected during the six days of oral dosing of nardosinone, and blood is collected at one hour after the last dose. Besides, to validate some metabolites, a fast experiment is performed, in which nardosinone was orally administered and the subsequent one-hour urine is collected and immediately analyzed by UHPLC-Q-TOF-MS. In total, 76 new metabolites are identified in this study, including 39, 51, and 12 metabolites in urine, plasma, and feces, respectively. Nardosinone can be converted into nardosinone acid or its isomers. The metabolic reactions of nardosinone included hydroxylation, hydrogenation, dehydration, glucuronidation, sulfation, demethylation, and carboxylation. There are 56 and 20 metabolites with the structural skeleton of nardosinone and nardosinone acid, respectively. In total, 77 in vivo existence forms of nardosinone are found in mice. Nardosinone is mainly excreted in urine and is not detected in the feces. These findings will lay the foundation for further research of the in vivo effective forms of nardosinone and Nardostachyos Radix et Rhizoma.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27217267