Structural basis of the mycobacterial stress-response RNA polymerase auto-inhibition via oligomerization

• Published in: Nature communications 2023-01, Vol.14 (1), p.484-484, Article 484
• Main Authors: Morichaud, Zakia, Trapani, Stefano, Vishwakarma, Rishi K., Chaloin, Laurent, Lionne, Corinne, Lai-Kee-Him, Joséphine, Bron, Patrick, Brodolin, Konstantin
• Format: Article
• Language: English
• Subjects: 101/28; 38/22; 38/70; 38/71; 631/326/421; 631/45/173; 631/535/1258/1259; 631/57/2272; 82/16; 82/80; 82/83; Allosteric properties; Bacterial Proteins - metabolism; Biological activity; Conformation; Cryoelectron Microscopy; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - metabolism; Electron microscopy; Gene expression; Hibernation; Holoenzymes - metabolism; Humanities and Social Sciences; Humans; Life Sciences; Macromolecules; multidisciplinary; Mycobacterium tuberculosis - genetics; Oligomerization; Ribonucleic acid; RNA; RNA polymerase; Science; Science (multidisciplinary); Self-assembly; Sigma Factor - metabolism; Transcription factors; Transcription Factors - metabolism; Transcription initiation; Transcription, Genetic
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-36113-y

Self-assembly of macromolecules into higher-order symmetric structures is fundamental for the regulation of biological processes. Higher-order symmetric structure self-assembly by the gene expression machinery, such as bacterial DNA-dependent RNA polymerase (RNAP), has never been reported before. Here, we show that the stress-response σ B factor from the human pathogen, Mycobacterium tuberculosis , induces the RNAP holoenzyme oligomerization into a supramolecular complex composed of eight RNAP units. Cryo-electron microscopy revealed a pseudo-symmetric structure of the RNAP octamer in which RNAP protomers are captured in an auto-inhibited state and display an open-clamp conformation. The structure shows that σ B is sequestered by the RNAP flap and clamp domains. The transcriptional activator RbpA prevented octamer formation by promoting the initiation-competent RNAP conformation. Our results reveal that a non-conserved region of σ is an allosteric controller of transcription initiation and demonstrate how basal transcription factors can regulate gene expression by modulating the RNAP holoenzyme assembly and hibernation. Biological processes can be regulated via oligomerization of macromolecules into high-order symmetric structures. Here, authors reported high-order structure of RNA polymerase and its role in regulation of gene expression in pathogenic bacterium.