Loading…
An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations
Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical developm...
Saved in:
Published in: | Disease models & mechanisms 2014-12, Vol.7 (12), p.1397-1405 |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03 |
---|---|
cites | cdi_FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03 |
container_end_page | 1405 |
container_issue | 12 |
container_start_page | 1397 |
container_title | Disease models & mechanisms |
container_volume | 7 |
creator | Kindberg, Abigail A Bendriem, Raphael M Spivak, Charles E Chen, Jia Handreck, Annelie Lupica, Carl R Liu, Jinny Freed, William J Lee, Chun-Ting |
description | Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical development, while providing new avenues for exploration of developmental neocortical abnormalities. Here, we show that preserving hPSCs aggregates - allowing embryoid body formation - while adding basic fibroblast growth factor (bFGF) during neuroepithelial development generates neural rosettes showing dorsal forebrain identity, including Mash1(+) dorsal telencephalic GABAergic progenitors. Structures that mirrored the organization of the cerebral cortex formed after rosettes were seeded and cultured for 3 weeks in the presence of FGF18, BDNF and NT3. Neurons migrated along radial glia scaffolding, with deep-layer CTIP2(+) cortical neurons appearing after 1 week and upper-layer SATB2(+) cortical neurons forming during the second and third weeks. At the end of differentiation, these structures contained both glutamatergic and GABAergic neurons, with glutamatergic neurons being most abundant. Thus, this differentiation protocol generated an hPSC-based model that exhibits temporal patterning and a neuronal subtype ratio similar to that of the developing human neocortex. This model was used to examine the effects of cocaine during neocorticogenesis. Cocaine caused premature neuronal differentiation and enhanced neurogenesis of various cortical neuronal subtypes. These cocaine-induced changes were inhibited by the cytochrome P450 inhibitor cimetidine. This in vitro model enables mechanistic studies of neocorticogenesis, and can be used to examine the mechanisms through which cocaine alters the development of the human neocortex. |
doi_str_mv | 10.1242/dmm.017251 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_f1e077aab17c47938a76f57a7c47904a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_f1e077aab17c47938a76f57a7c47904a</doaj_id><sourcerecordid>2688529972</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03</originalsourceid><addsrcrecordid>eNpVkUtv1DAUhSMEoqWw4QcgS-yQUvxIYpsFUlVBqVSJDaytGz9mPErsYDsjdcNvx9Mpo3Zl-_r4u_f4NM17gi8J7ehnM8-XmHDakxfNOeF914qOkJenPWZnzZucdxgPVDD5ujmjPRViEPS8-XsVkA9o70uKaI7GTig6tF1nCCjYqGMqXsOEjN3bKS6zDQWt2YcNWqY1-SWWQyUXOyNtpyl_QTpq8MG2PphVW4P0fYmQ9NYXq8uaKgumYhMUH0N-27xyMGX77nG9aH5___br-kd79_Pm9vrqrtUdG0pLDRspsZgwGA1I7KyU0hGgmgkpyAiic05LogV2XIBj3JCOjFZ3XBIKmF00t0euibBTS_IzpHsVwauHQkwbBQenk1Wu9uEcYCT88JwJ4IPrOTyccAeV9fXIWtZxtkbXD6iunkGf3wS_VZu4Vx3tOcaiAj4-AlL8s9pc1C6uKVT_ig5C9FRKTqvq01GlU8w5WXfqQLA65K5q7uqYexV_eDrTSfo_aPYPIrKspg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2688529972</pqid></control><display><type>article</type><title>An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Kindberg, Abigail A ; Bendriem, Raphael M ; Spivak, Charles E ; Chen, Jia ; Handreck, Annelie ; Lupica, Carl R ; Liu, Jinny ; Freed, William J ; Lee, Chun-Ting</creator><creatorcontrib>Kindberg, Abigail A ; Bendriem, Raphael M ; Spivak, Charles E ; Chen, Jia ; Handreck, Annelie ; Lupica, Carl R ; Liu, Jinny ; Freed, William J ; Lee, Chun-Ting</creatorcontrib><description>Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical development, while providing new avenues for exploration of developmental neocortical abnormalities. Here, we show that preserving hPSCs aggregates - allowing embryoid body formation - while adding basic fibroblast growth factor (bFGF) during neuroepithelial development generates neural rosettes showing dorsal forebrain identity, including Mash1(+) dorsal telencephalic GABAergic progenitors. Structures that mirrored the organization of the cerebral cortex formed after rosettes were seeded and cultured for 3 weeks in the presence of FGF18, BDNF and NT3. Neurons migrated along radial glia scaffolding, with deep-layer CTIP2(+) cortical neurons appearing after 1 week and upper-layer SATB2(+) cortical neurons forming during the second and third weeks. At the end of differentiation, these structures contained both glutamatergic and GABAergic neurons, with glutamatergic neurons being most abundant. Thus, this differentiation protocol generated an hPSC-based model that exhibits temporal patterning and a neuronal subtype ratio similar to that of the developing human neocortex. This model was used to examine the effects of cocaine during neocorticogenesis. Cocaine caused premature neuronal differentiation and enhanced neurogenesis of various cortical neuronal subtypes. These cocaine-induced changes were inhibited by the cytochrome P450 inhibitor cimetidine. This in vitro model enables mechanistic studies of neocorticogenesis, and can be used to examine the mechanisms through which cocaine alters the development of the human neocortex.</description><identifier>ISSN: 1754-8403</identifier><identifier>EISSN: 1754-8411</identifier><identifier>DOI: 10.1242/dmm.017251</identifier><identifier>PMID: 25288682</identifier><language>eng</language><publisher>England: The Company of Biologists Ltd</publisher><subject>Cell Differentiation ; Cell Line ; Cerebral Cortex - embryology ; Cocaine ; Cocaine - chemistry ; Cytochrome ; Dopamine Uptake Inhibitors - chemistry ; Dorsal forebrain model ; Fibroblast Growth Factor 2 - metabolism ; hPSCs ; Humans ; Immunohistochemistry ; Mammals ; Metabolism ; Neocortical development ; Neurogenesis ; Neurons ; Neurons - metabolism ; Oxidative stress ; Pluripotent Stem Cells - cytology ; Premature neuronal differentiation ; Prosencephalon - embryology ; Reactive Oxygen Species ; Resource ; Stem cells ; Stem Cells - metabolism</subject><ispartof>Disease models & mechanisms, 2014-12, Vol.7 (12), p.1397-1405</ispartof><rights>2014. Published by The Company of Biologists Ltd.</rights><rights>2014. This work is licensed under http://creativecommons.org/licenses/by/3.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014. Published by The Company of Biologists Ltd 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03</citedby><cites>FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2688529972/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2688529972?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25288682$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kindberg, Abigail A</creatorcontrib><creatorcontrib>Bendriem, Raphael M</creatorcontrib><creatorcontrib>Spivak, Charles E</creatorcontrib><creatorcontrib>Chen, Jia</creatorcontrib><creatorcontrib>Handreck, Annelie</creatorcontrib><creatorcontrib>Lupica, Carl R</creatorcontrib><creatorcontrib>Liu, Jinny</creatorcontrib><creatorcontrib>Freed, William J</creatorcontrib><creatorcontrib>Lee, Chun-Ting</creatorcontrib><title>An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations</title><title>Disease models & mechanisms</title><addtitle>Dis Model Mech</addtitle><description>Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical development, while providing new avenues for exploration of developmental neocortical abnormalities. Here, we show that preserving hPSCs aggregates - allowing embryoid body formation - while adding basic fibroblast growth factor (bFGF) during neuroepithelial development generates neural rosettes showing dorsal forebrain identity, including Mash1(+) dorsal telencephalic GABAergic progenitors. Structures that mirrored the organization of the cerebral cortex formed after rosettes were seeded and cultured for 3 weeks in the presence of FGF18, BDNF and NT3. Neurons migrated along radial glia scaffolding, with deep-layer CTIP2(+) cortical neurons appearing after 1 week and upper-layer SATB2(+) cortical neurons forming during the second and third weeks. At the end of differentiation, these structures contained both glutamatergic and GABAergic neurons, with glutamatergic neurons being most abundant. Thus, this differentiation protocol generated an hPSC-based model that exhibits temporal patterning and a neuronal subtype ratio similar to that of the developing human neocortex. This model was used to examine the effects of cocaine during neocorticogenesis. Cocaine caused premature neuronal differentiation and enhanced neurogenesis of various cortical neuronal subtypes. These cocaine-induced changes were inhibited by the cytochrome P450 inhibitor cimetidine. This in vitro model enables mechanistic studies of neocorticogenesis, and can be used to examine the mechanisms through which cocaine alters the development of the human neocortex.</description><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cerebral Cortex - embryology</subject><subject>Cocaine</subject><subject>Cocaine - chemistry</subject><subject>Cytochrome</subject><subject>Dopamine Uptake Inhibitors - chemistry</subject><subject>Dorsal forebrain model</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>hPSCs</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Mammals</subject><subject>Metabolism</subject><subject>Neocortical development</subject><subject>Neurogenesis</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Oxidative stress</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Premature neuronal differentiation</subject><subject>Prosencephalon - embryology</subject><subject>Reactive Oxygen Species</subject><subject>Resource</subject><subject>Stem cells</subject><subject>Stem Cells - metabolism</subject><issn>1754-8403</issn><issn>1754-8411</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpVkUtv1DAUhSMEoqWw4QcgS-yQUvxIYpsFUlVBqVSJDaytGz9mPErsYDsjdcNvx9Mpo3Zl-_r4u_f4NM17gi8J7ehnM8-XmHDakxfNOeF914qOkJenPWZnzZucdxgPVDD5ujmjPRViEPS8-XsVkA9o70uKaI7GTig6tF1nCCjYqGMqXsOEjN3bKS6zDQWt2YcNWqY1-SWWQyUXOyNtpyl_QTpq8MG2PphVW4P0fYmQ9NYXq8uaKgumYhMUH0N-27xyMGX77nG9aH5___br-kd79_Pm9vrqrtUdG0pLDRspsZgwGA1I7KyU0hGgmgkpyAiic05LogV2XIBj3JCOjFZ3XBIKmF00t0euibBTS_IzpHsVwauHQkwbBQenk1Wu9uEcYCT88JwJ4IPrOTyccAeV9fXIWtZxtkbXD6iunkGf3wS_VZu4Vx3tOcaiAj4-AlL8s9pc1C6uKVT_ig5C9FRKTqvq01GlU8w5WXfqQLA65K5q7uqYexV_eDrTSfo_aPYPIrKspg</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Kindberg, Abigail A</creator><creator>Bendriem, Raphael M</creator><creator>Spivak, Charles E</creator><creator>Chen, Jia</creator><creator>Handreck, Annelie</creator><creator>Lupica, Carl R</creator><creator>Liu, Jinny</creator><creator>Freed, William J</creator><creator>Lee, Chun-Ting</creator><general>The Company of Biologists Ltd</general><general>The Company of Biologists Limited</general><general>The Company of Biologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141201</creationdate><title>An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations</title><author>Kindberg, Abigail A ; Bendriem, Raphael M ; Spivak, Charles E ; Chen, Jia ; Handreck, Annelie ; Lupica, Carl R ; Liu, Jinny ; Freed, William J ; Lee, Chun-Ting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cerebral Cortex - embryology</topic><topic>Cocaine</topic><topic>Cocaine - chemistry</topic><topic>Cytochrome</topic><topic>Dopamine Uptake Inhibitors - chemistry</topic><topic>Dorsal forebrain model</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>hPSCs</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Mammals</topic><topic>Metabolism</topic><topic>Neocortical development</topic><topic>Neurogenesis</topic><topic>Neurons</topic><topic>Neurons - metabolism</topic><topic>Oxidative stress</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Premature neuronal differentiation</topic><topic>Prosencephalon - embryology</topic><topic>Reactive Oxygen Species</topic><topic>Resource</topic><topic>Stem cells</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kindberg, Abigail A</creatorcontrib><creatorcontrib>Bendriem, Raphael M</creatorcontrib><creatorcontrib>Spivak, Charles E</creatorcontrib><creatorcontrib>Chen, Jia</creatorcontrib><creatorcontrib>Handreck, Annelie</creatorcontrib><creatorcontrib>Lupica, Carl R</creatorcontrib><creatorcontrib>Liu, Jinny</creatorcontrib><creatorcontrib>Freed, William J</creatorcontrib><creatorcontrib>Lee, Chun-Ting</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Disease models & mechanisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kindberg, Abigail A</au><au>Bendriem, Raphael M</au><au>Spivak, Charles E</au><au>Chen, Jia</au><au>Handreck, Annelie</au><au>Lupica, Carl R</au><au>Liu, Jinny</au><au>Freed, William J</au><au>Lee, Chun-Ting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations</atitle><jtitle>Disease models & mechanisms</jtitle><addtitle>Dis Model Mech</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>7</volume><issue>12</issue><spage>1397</spage><epage>1405</epage><pages>1397-1405</pages><issn>1754-8403</issn><eissn>1754-8411</eissn><abstract>Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical development, while providing new avenues for exploration of developmental neocortical abnormalities. Here, we show that preserving hPSCs aggregates - allowing embryoid body formation - while adding basic fibroblast growth factor (bFGF) during neuroepithelial development generates neural rosettes showing dorsal forebrain identity, including Mash1(+) dorsal telencephalic GABAergic progenitors. Structures that mirrored the organization of the cerebral cortex formed after rosettes were seeded and cultured for 3 weeks in the presence of FGF18, BDNF and NT3. Neurons migrated along radial glia scaffolding, with deep-layer CTIP2(+) cortical neurons appearing after 1 week and upper-layer SATB2(+) cortical neurons forming during the second and third weeks. At the end of differentiation, these structures contained both glutamatergic and GABAergic neurons, with glutamatergic neurons being most abundant. Thus, this differentiation protocol generated an hPSC-based model that exhibits temporal patterning and a neuronal subtype ratio similar to that of the developing human neocortex. This model was used to examine the effects of cocaine during neocorticogenesis. Cocaine caused premature neuronal differentiation and enhanced neurogenesis of various cortical neuronal subtypes. These cocaine-induced changes were inhibited by the cytochrome P450 inhibitor cimetidine. This in vitro model enables mechanistic studies of neocorticogenesis, and can be used to examine the mechanisms through which cocaine alters the development of the human neocortex.</abstract><cop>England</cop><pub>The Company of Biologists Ltd</pub><pmid>25288682</pmid><doi>10.1242/dmm.017251</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1754-8403 |
ispartof | Disease models & mechanisms, 2014-12, Vol.7 (12), p.1397-1405 |
issn | 1754-8403 1754-8411 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_f1e077aab17c47938a76f57a7c47904a |
source | Publicly Available Content Database; PubMed Central |
subjects | Cell Differentiation Cell Line Cerebral Cortex - embryology Cocaine Cocaine - chemistry Cytochrome Dopamine Uptake Inhibitors - chemistry Dorsal forebrain model Fibroblast Growth Factor 2 - metabolism hPSCs Humans Immunohistochemistry Mammals Metabolism Neocortical development Neurogenesis Neurons Neurons - metabolism Oxidative stress Pluripotent Stem Cells - cytology Premature neuronal differentiation Prosencephalon - embryology Reactive Oxygen Species Resource Stem cells Stem Cells - metabolism |
title | An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T05%3A48%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20in%20vitro%20model%20of%20human%20neocortical%20development%20using%20pluripotent%20stem%20cells:%20cocaine-induced%20cytoarchitectural%20alterations&rft.jtitle=Disease%20models%20&%20mechanisms&rft.au=Kindberg,%20Abigail%20A&rft.date=2014-12-01&rft.volume=7&rft.issue=12&rft.spage=1397&rft.epage=1405&rft.pages=1397-1405&rft.issn=1754-8403&rft.eissn=1754-8411&rft_id=info:doi/10.1242/dmm.017251&rft_dat=%3Cproquest_doaj_%3E2688529972%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c436t-2d3b21e013abda90fe999f1a2c38981ba84ffc91c80f78af37d141bec47912a03%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2688529972&rft_id=info:pmid/25288682&rfr_iscdi=true |